Results 61 to 70 of about 228,275 (300)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Colorimetric detection based on gold nano particles (GNPs): An easy, fast, inexpensive, low-cost and short time method in detection of analytes (protein, DNA, and ion)

Sensing and Bio-Sensing Research, 2018
Gold nanoparticles (GNPs), due to their unique physical and chemical properties, including optical properties, are highly regarded and have extensive applications in biomedical.
Mir Hadi Jazayeri, Tayebe Aghaie, Amir Avan, Ali Vatankhah, Mohmad Reza S. Ghaffari   +4 more
doaj   +1 more source

Anthocyanins of Açaí Applied as a Colorimetric Indicator of Milk Spoilage: A Study Using Agar-Agar and Cellulose Acetate as Solid Support to Be Applied in Packaging

Polysaccharides, 2022
Food that is still fit for consumption is wasted in the domestic environment every day, so food packaging technologies are being developed that will monitor the quality of the products in real time. Highly perishable milk is currently one of the products
Samiris Côcco Teixeira, Taila Veloso de Oliveira, Lais Fernanda Batista, Rafael Resende Assis Silva, Matheus de Paula Lopes, Alane Rafaela Costa Ribeiro, Thaís Caroline Buttow Rigolon, Paulo César Stringheta, Nilda de Fátima Ferreira Soares   +8 more
doaj   +1 more source

Development and deployment of a microfluidic platform for water quality monitoring [PDF]

, 2013
There is an increasing demand for autonomous sensor devices which can provide reliable data on key water quality parameters at a higher temporal and geographical resolution than is achievable using current approaches to sampling and monitoring ...
Cleary, John, Diamond, Dermot, Maher, Damien   +2 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Colorimetric Analysis of Transmitted Light Through Plasmonic Paper for Next-Generation Point-of-Care (PoC) Devices

Biosensors
This study identifies the optimal conditions for enhancing the performance of the Color Picker System, a device designed for colorimetric sensing using plasmonic paper.
Caterina Serafinelli, Alessandro Fantoni, Elisabete C. B. A. Alegria, Manuela Vieira   +3 more
doaj   +1 more source

Erythrosin B: a versatile colorimetric and fluorescent vital dye for bacteria

BioTechniques, 2020
Rapidly assaying cell viability for diverse bacteria species is not always straightforward. In eukaryotes, cell viability is often determined using colorimetric dyes; however, such dyes have not been identified for bacteria.
Josef D Franke, Ann L Braverman, Alison M Cunningham, Erin E Eberhard, Greg A Perry   +4 more
doaj   +1 more source

Optimization Of Detergent-Mediated Reconstitution Of Influenza A M2 Protein Into Proteoliposomes [PDF]

, 2018
We report the optimization of detergent-mediated reconstitution of an integral membrane-bound protein, full-length influenza M2 protein, by direct insertion into detergent-saturated liposomes.
Arbuckle, Douglas Stuart, , \u2717, Bost, Margaret H., , \u2717, Crouch, Catherine Hirshfeld, Green, Bryan M., , \u2716, Grossman, Carl H., Howard, Kathleen P., Kim, Tae H., , \u2714   +6 more
core   +2 more sources

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Colorimetric Biosensors: Advancements in Nanomaterials and Cutting-Edge Detection Strategies

Biosensors
Colorimetric-based biosensors are practical detection devices that can detect the presence and concentration of biomarkers through simple color changes.
Yubeen Lee, Izzati Haizan, Sang Baek Sim, Jin-Ha Choi   +3 more
doaj   +1 more source