Results 31 to 40 of about 29,130 (259)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Effects of colour and scene dynamism on visual fatigue in animated films

Scientific Reports
Animated films are highly popular in society, particularly among children and adolescents. During film production, selecting an adequate colour palette and scene dynamism is crucial for creating a visual impact on the audience.
Marc Argilés, Elisabet Fonts, Luis Pérez-Mañá, Beatriz Martinez-Navarro, Carles Sora-Domenjó, Elisabet Pérez-Cabré, Bernat Sunyer-Grau, Cristina Rovira-Gay, Carla Molins-Pitarch, Lluïsa Quevedo-Junyent   +9 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

From the Margins to the Mainstream? The Eastmancolor Revolution and Challenging the Realist Canon in British Cinema

Cinéma & Cie, 2019
This article focuses on Eastmancolor’s introduction in the British film industry from 1954 to the complete adoption of colour by the end of the 1960s.
Sarah Street, Keith M. Johnston, Paul Frith, Carolyn Rickards   +3 more
doaj  

A pseudo‐colour enhancement algorithm for high‐bit RAW greyscale image of X‐ray film displaying on low‐bit monitors

IET Image Processing, 2023
X‐ray flaw detection is widely used in non‐destructive area. The intuitive defect information can be obtained through the X‐ray film, which is usually digitized into high greyscale image by a 12‐bit or 16‐bit, called super 8‐bit, industrial scanner. When
Zhigang Lv, Liangliang Li, Hongxi Wang, Peng Wang, Jianheng Li, Lei Shu, Xiaoyan Li   +6 more
doaj   +1 more source

Influence of Wet Film Thickness on the Functional Applications of Biopolymers in Paper Conservation

Colloids and Interfaces, 2023
In heritage conservation, paper deterioration can be slowed down by controlling the environmental conditions surrounding heritage objects and stabilizing the materials these objects are made of. As conservation materials can also cause optical, chemical,
Gabriela Aleksić, Tomislav Cigula, Marina Vukoje, Katarina Itrić Ivanda   +3 more
doaj   +1 more source

Plasma EV Proteomics Identifies ECM Remodeling and Inflammatory Proteins LUM and C7 as Candidate Biomarkers in FSHD

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Facioscapulohumeral muscular dystrophy (FSHD) is one of the most debilitating and common muscular dystrophies. Despite its severity, no approved therapy exists for FSHD patients. However, several therapeutic candidates are currently under development, and some have recently entered clinical trials, marking the need for reliable ...
Mustafa Bilal Bayazit, Chiranth K. Nagaraj, Jackson S. Newell, Kim Truc Nguyen, Xilal Y. Rima, Jacob Doon‐Ralls, Eduardo Reátegui, Jeffrey M. Statland, Rabi Tawil, Kevin M. Flanigan, Scott Q. Harper, Nizar Y. Saad   +11 more
wiley   +1 more source

Harnessing machine learning for chicken meat quality evaluation using curcumin-loaded methylcellulose intelligent films [PDF]

Sustainable Food Technology
In this study, curcumin (Cur) was incorporated into a methylcellulose (MC) biopolymer to develop an intelligent packaging system for real-time monitoring of chicken meat freshness (at 4 ± 2 °C), using machine learning (ML). Artificial neural network (ANN)
Suresh Chandra Varsha, Ashique Thazheparamban, Arunkumar Panneerselvam   +2 more
doaj   +1 more source