Results 91 to 100 of about 1,579,148 (310)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Perceptions of Lecturers, Administrators, and Students About the Workplace as Learning Environment for Undergraduate Medical Students at a National Referral and Teaching Hospital in Uganda

Advances in Medical Education and Practice, 2022
Mike Nantamu Kagawa,1 Sarah Kiguli,2 Hannes Steinberg,3 Mpho Priscilla Jama4 1Department of Obstetrics & Gynaecology, Makerere University, Kampala, Uganda; 2Department of Paediatrics & Child health, Makerere University, Kampala, Uganda; 3Department of ...
Kagawa MN, Kiguli S, Steinberg H, Jama MP   +3 more
doaj  

Beyond competence: digital literacies as knowledge practices, and implications for learner development [PDF]

, 2012
Our interest in studying digital literacies arises from what we perceive as a failure to develop students' capacities to learn deeply in a technology-rich environment. The trends towards networked communities and digital citizenship, as well as workplace
Beetham, Helen, Littlejohn, Allison, McGill, Lou   +2 more
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

An Ontological-based Model for Competences in Sustainable Development Projects: a Case Study for Project’s Commercial Activities [PDF]

The paper presents a project management competencies model, using an ontological approach. The ontology, named PMCatalog, was developed in the framework of the project CONTO, financed by Romanian through the grant 91-037/2007.
Ana Tănăsescu, Cetin Elmas, Constanţa-Nicoleta Bodea, Maria Dascălu   +3 more
core  

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

FlightScope: An Experimental Comparative Review of Aircraft Detection Algorithms in Satellite Imagery

Remote Sensing
Object detection in remotely sensed satellite pictures is fundamental in many fields such as biophysical and environmental monitoring. While deep learning algorithms are constantly evolving, they have been mostly implemented and tested on popular ground ...
Safouane El Ghazouali, Arnaud Gucciardi, Francesca Venturini, Nicola Venturi, Michael Rueegsegger, Umberto Michelucci   +5 more
doaj   +1 more source

Early signatures of regime shifts in gene expression dynamics

, 2013
Recently, a large number of studies have been carried out on the early signatures of sudden regime shifts in systems as diverse as ecosystems, financial markets, population biology and complex diseases.
Bose, Indrani, Ghosh, Sayantari, Pal, Amit Kumar, Pal, Mainak   +3 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source