Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A psychological model of competitive behavior: social comparison as a mediator of the critical thinking, self-efficacy, and adaptation ability prediction among college students. [PDF]
Heliyon, 2022 Mildawani MMTS +3 more
europepmc +1 more source
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
The causal effect of testosterone on men's competitive behavior is moderated by basal cortisol and cues to an opponent's status: Evidence for a context-dependent dual-hormone hypothesis. [PDF]
J Pers Soc Psychol, 2022 Knight EL +6 more
europepmc +1 more source
The Visible Hand: Finger Ratio (2D:4D) and Competitive Behavior [PDF]
In an experiment using two-bidder first-price sealed bid auctions with symmetric independent private values, we scan also the right hand of each subject.
Burkhard C. Schipper, Matthew Pearson
core
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Firm Organization and Retail Industry Dynamics [PDF]
This paper investigates the spatial organization and dynamics of retail markets using establishment level data on entry, exit, and location choice in the retail alcoholic beverage industry.
Emin M. Dinlersoz
core
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
FEBS Open Bio, EarlyView.During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source
Supplemental Notes to "Demographic transition and industrial revolution: A macroeconomic investigation" [PDF]
These notes cover: Derivation of the objective function of the Dynastic Problem, Characterization of the competitive equilibrium, Sequential Problem (SP) whose solution corresponds to the competitive equilibrium allocation, Limiting behavior of the ...
Michael Bar, Oksana Leukhina
core