Results 301 to 310 of about 285,043 (352)

Competitive protein binding assay of corticosterone

Journal of Steroid Biochemistry, 1972
Abstract Details of a competitive protein binding assay of corticosterone are described. The preliminary TLC separation permits simultaneous estimation of corticosterone, aldosterone and progesterone. “Unstressed” rat plasma corticosterone values were obtained between 0.3 and 9 μg/ 100 ml.
A, Spät, S, Józan
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Effect of transport and competition on ligand binding

Biophysical Chemistry, 1999
We present a model to describe the physics of chemoreception in processes determined by competitive ligand binding. Our model describes the competition between various populations, such as ligands vs. blockers and receptors vs. decoys, in protein activation when diffusion is rate-determining.
C. A. CONDAT, DELSANTO, PIER PAOLO, E. RUFFINO, G. PEREGO   +3 more
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Cefmenoxime and Bilirubin: Competition for Albumin Binding

Pharmacology & Toxicology, 1991
Abstract:Certain drugs are known to compete with bilirubin for albumin binding; therefore, all drugs administered to neonates should be tested to determine the degree of competition. The effect of cefmenoxime on bilirubin‐albumin binding was determined by comparing the oxidation rate of free bilirubin in the presence and absence of drug.
D L, Onks, J F, Harris, A F, Robertson
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ASSAY OF ALDOSTERONE BY COMPETITIVE PROTEIN BINDING

Acta Endocrinologica, 1970
ABSTRACT We have applied a competitive protein binding technique to the assay of aldosterone, utilizing the aldosterone binding protein (ABP) in kidney as the specific receptor molecule. Intracellular ABP was obtained from the homogenization of adrenalectomized rat kidneys in 0.25 M sucrose.
R G, Robinson, D D, Fanestil
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Competitive Protein Binding Assay for Piritrexim

Journal of Pharmaceutical Sciences, 1989
A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by
J L, Woolley, J L, Ringstad, C W, Sigel
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Determination of calmodulin by competitive binding assay

Analytical Biochemistry, 1986
Calmodulin levels in tissue or cellular extracts can be determined by competition with 125I-calmodulin in a filtration-based direct binding assay. The method is rapid, uses readily available stable components, and possesses a selectivity and sensitivity comparable to that observed with immunoassay and phosphodiesterase activation. This assay provides a
H, LeVine, N, Sahyoun, P, Cuatrecasas
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Measurement of thyroxine by competitive protein binding

Clinica Chimica Acta, 1972
Abstract A simplified method is described for the measurement of thyroxine by competitive protein binding, using Sephadex columns for separation of bound from unbound thyroxine. Conditions for the various steps in the method were studied. The results compare well with PBI determinations done on the same subjects.
H, Seligson, D, Seligson
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Competitive protein binding assay for 24,25-dihydroxycholecalciferol

Biochemical and Biophysical Research Communications, 1976
Abstract A competitive protein binding assay which measures 24,25-dihydroxycholecalciferol in human serum has been developed using the binding protein from vitamin D-deficient rat kidney. As 25-hydroxycholecalciferol and 25,26-dihydroxycholecalciferol also interact with the binding protein, possible interference by these compounds in the assay has ...
C M, Taylor, S E, Hughes, P, de Silva
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The Problem of Partial Competition in the Quantitative Characterization of Interactions by Competitive Binding Assays

Analytical Biochemistry, 1993
Binding expressions are derived and analytical procedures developed for the quantitative characterization of inhibitor binding that is only partially competitive with the interaction between an acceptor and the ligand that is being monitored. Two such situations are considered: (i) that in which the partial competition reflects binding of inhibitor to ...
Brocklebank, Anne M., Sawyer, William H., Wiley, James S., Winzor, Donald J.   +3 more
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Bargaining, binding contracts, and competitive wages

Games and Economic Behavior, 2003
In a model where many workers bargain with one firm and sign binding contracts, the existence of a stationary subgame perfect equilibrium is shown. If the production function satisfies decreasing returns, each worker receives a share of his marginal product (treating all other workers as employed) in equilibrium. Thus, wages are competitive. This is in
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