Results 231 to 240 of about 48,799 (265)
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Effects of activated complement components on enzyme secretion by macrophages.
Immunology, 1977Purified cleavage products of the guinea-pig complement component C3, namely C3b and C3a, interact with guinea-pig and mouse macrophages in culture to induce a dose- and time dependent release of lysosmal enzymes into the medium. In the case of C3b the selectivity of the release of hydrolases, which occurs without cell killing, is shown by ...
H U, Schorlemmer, A C, Allison
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Digestion of the fifth component of complement by eosinophil lysosomal enzymes
Virchows Archiv B Cell Pathology Including Molecular Pathology, 1981Recently our laboratory has shown that neutrophils contain enzymatic activity within their lysosomal granules which will generate chemotactic activity for neutrophils and tumor cells from the fifth component of complement (C5). We have now expanded this initial observation and have demonstrated that eosinophils can release enzymatic activity from their
H, Ogawa +3 more
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
Three ataxia telangiectasia homozygotes, one heterozygote and normal fibroblast strains were compared as to the capacity of their cellular extracts to enhance the priming activity of gamma-irradiated colicin E1 DNA for purified DNA polymerase (EC 2.7.7.7) of Escherichia coli.
T, Inoue, A, Yokoiyama, T, Kada
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Three ataxia telangiectasia homozygotes, one heterozygote and normal fibroblast strains were compared as to the capacity of their cellular extracts to enhance the priming activity of gamma-irradiated colicin E1 DNA for purified DNA polymerase (EC 2.7.7.7) of Escherichia coli.
T, Inoue, A, Yokoiyama, T, Kada
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Biochimica et Biophysica Acta (BBA) - Enzymology, 1973
Abstract The relation of purified C1sa to other plasma enzymes was investigated. C1sa does not activate purified plasminogen or plasma kallikreinogen. No conversion of proenzyme C1s to C1sa was observed on incubation of purified C1s alone or with purified C1sa, but addition of C1r (one of the three subunits of C1) caused rapid conversion of C1s to ...
Hiroyuki Sumi +2 more
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Abstract The relation of purified C1sa to other plasma enzymes was investigated. C1sa does not activate purified plasminogen or plasma kallikreinogen. No conversion of proenzyme C1s to C1sa was observed on incubation of purified C1s alone or with purified C1sa, but addition of C1r (one of the three subunits of C1) caused rapid conversion of C1s to ...
Hiroyuki Sumi +2 more
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[Description of an unusual enzymic activity cleaving the third component of complement].
Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles, 1979A large enzymatic complex cleaving C3 (C3'ase) in the absence of magnesium (EDTA) has been partially characterized in two patients. On gel filtration EDTA-C3'ase was found in an 800 000 fraction containing antigenic C4, IgA and IgG in addition to alpha-2-macroglobulin and IgM normally present.
J, Leibowitch, M, Leveille, L, Halbwachs
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2012
The recruitment of arrestins to activated 7TMRs results in the activation of alternative signaling pathways, quenching of G-protein activation, and coupling to clathrin-mediated endocytosis. The nearly ubiquitous involvement of arrestin in 7TMR signaling has spurred the development of several methods for monitoring this interaction in mammalian cells ...
Daniel L, Bassoni +4 more
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The recruitment of arrestins to activated 7TMRs results in the activation of alternative signaling pathways, quenching of G-protein activation, and coupling to clathrin-mediated endocytosis. The nearly ubiquitous involvement of arrestin in 7TMR signaling has spurred the development of several methods for monitoring this interaction in mammalian cells ...
Daniel L, Bassoni +4 more
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ASSAY and Drug Development Technologies, 2006
The majority of protein kinase assays used in drug discovery research are enzyme activity assays. These assays are based on the measurement of phosphorylated protein or peptide substrate, which is the end product of the enzyme reaction. Since most kinase inhibitors are ATP competitive, prediction of the activity of compounds in cellular systems based ...
Guido J R, Zaman +4 more
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The majority of protein kinase assays used in drug discovery research are enzyme activity assays. These assays are based on the measurement of phosphorylated protein or peptide substrate, which is the end product of the enzyme reaction. Since most kinase inhibitors are ATP competitive, prediction of the activity of compounds in cellular systems based ...
Guido J R, Zaman +4 more
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European Journal of Immunology, 1971
AbstractAn enzymatic complex can be formed by factors from guinea pig serum and cobra venom, which is able to activate C3 bypassing C1, C4 and C2. Formation and action of the enzyme are described. The action on C3 results in an activation of the terminal complement components and in membrane destruction provided suitable membrane receptors are ...
M P, Dierich +3 more
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AbstractAn enzymatic complex can be formed by factors from guinea pig serum and cobra venom, which is able to activate C3 bypassing C1, C4 and C2. Formation and action of the enzyme are described. The action on C3 results in an activation of the terminal complement components and in membrane destruction provided suitable membrane receptors are ...
M P, Dierich +3 more
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Mutation Research, 1971
Abstract Polar-complementing pyrimidine-3 mutants that lack both carbamyl phosphate synthetase and asparate transcarbamylase were tested for their ability to have either of their 2 functions restored by mutation. Such partial reversions restoring only the aspartate transcarbamylase function were recovered from some, but not all, of the alleles tested.
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Abstract Polar-complementing pyrimidine-3 mutants that lack both carbamyl phosphate synthetase and asparate transcarbamylase were tested for their ability to have either of their 2 functions restored by mutation. Such partial reversions restoring only the aspartate transcarbamylase function were recovered from some, but not all, of the alleles tested.
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Journal of Microbiological Methods, 2000
A method is described which allows the evaluation of the membrane lytic activity of either complement or antimicrobial peptides against the extracellular stage of the human protozoan parasite Toxoplasma gondii. The assay is based on lacZ transgenic parasites, determining the activity of released cytoplasmic beta-galactosidase into the culture ...
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A method is described which allows the evaluation of the membrane lytic activity of either complement or antimicrobial peptides against the extracellular stage of the human protozoan parasite Toxoplasma gondii. The assay is based on lacZ transgenic parasites, determining the activity of released cytoplasmic beta-galactosidase into the culture ...
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