Results 101 to 110 of about 801,714 (279)

Accommodation of Helical Imperfections in Rhodobacter sphaeroides Argonaute Ternary Complexes with Guide RNA and Target DNA

Cell Reports, 2018
Summary: Prokaryotic Argonaute (Ago) proteins were recently shown to target foreign genetic elements, thus making them a perfect model for studies of interference mechanisms.
Yiwei Liu, Daria Esyunina, Ivan Olovnikov, Marianna Teplova, Andrey Kulbachinskiy, Alexei A. Aravin, Dinshaw J. Patel   +6 more
doaj   +1 more source

PARP inhibitors elicit distinct transcriptional programs in homologous recombination competent castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham, Jasibel Vasquez‐Gonzalez, Samantha M. Barnada, Salome Tchotorlishvili, Latese Jones, Ryan Maguire, Genevieve Lewis, Kinza Rizwan, Jenny Deng, Salma Koachar, Drithi Patel, Hailey Shankle, Tessa Mulders, Namra Ajmal, Charalambos Solomides, Emad S. Alnemri, Teresa F. Alnemri, Ayesha A. Shafi, Leonard G. Gomella, Wm Kevin Kelly, Steven B. McMahon, Matthew J. Schiewer   +21 more
wiley   +1 more source

POSoligo software for in vitro gene synthesis

Scientific Reports
Oligonucleotide synthesis is vital for molecular experiments. Bioinformatics has been employed to create various algorithmic tools for the in vitro synthesis of nucleotides.
Yingying Tong, Jie Sun, Yang Chen, Changhua Yi, Hua Wang, Caixin Li, Nana Dai, Guanghua Yang   +7 more
doaj   +1 more source

Data analysis of viral complementary DNA/RNA sequences for low-frequency intrahost-single nucleotide variants in COVID-19. [PDF]

Proc Natl Acad Sci U S A, 2022
Chandra S.
europepmc   +1 more source

Characterizing the salivary RNA landscape to identify potential diagnostic, prognostic, and follow‐up biomarkers for breast cancer

Molecular Oncology, EarlyView.
This study explores salivary RNA for breast cancer (BC) diagnosis, prognosis, and follow‐up. High‐throughput RNA sequencing identified distinct salivary RNA signatures, including novel transcripts, that differentiate BC from healthy controls, characterize histological and molecular subtypes, and indicate lymph node involvement.
Nicholas Rajan, Irina Primac, Emre Etlioglu, Laurens Debruyne, Ann Janssen, Magy Sallam, Kevin Tabury, Roel Quintens, Wiebren Tjalma, Mohammed Abderrafi Benotmane   +9 more
wiley   +1 more source

Locked nucleic acid inhibits amplification of contaminating DNA in real-time PCR

BioTechniques, 2005
Locked nucleic acid (LNA®) is a modified DNA with increased binding affinity for complementary DNA sequences. Our strategy was to use this property of LNA to inhibit undesired PCR amplification (e.g., from contaminating genomic DNA) in a cDNA-based assay.
Lone Hummelshoj, Lars P. Ryder, Hans O. Madsen, Lars K. Poulsen   +3 more
doaj   +1 more source

Rescue of the first alphanucleorhabdovirus entirely from cloned complementary DNA: An efficient vector for systemic expression of foreign genes in maize and insect vectors. [PDF]

Mol Plant Pathol, 2023
Kanakala S, Xavier CAD, Martin KM, Tran HH, Redinbaugh MG, Whitfield AE.   +5 more
europepmc   +1 more source

The process of infection with bacteriophage phiX174, XXII. Synthesis of progeny single-stranded DNA [PDF]

, 1968
In a previous article,(1) it was demonstrated that of the labeled thymidine incorporated into the replicative form (RF) of phiX-DNA during the period of RF replication (3-12 or 15 min after infection), approximately half was transferred to virus during ...
Knippers, Rolf, Komano, Tohru, Sinsheimer, Robert L.   +2 more
core  

Bridging the gap: Multi‐stakeholder perspectives of molecular diagnostics in oncology

Molecular Oncology, EarlyView.
Although molecular diagnostics is transforming cancer care, implementing novel technologies remains challenging. This study identifies unmet needs and technology requirements through a two‐step stakeholder involvement. Liquid biopsies for monitoring applications and predictive biomarker testing emerge as key unmet needs. Technology requirements vary by
Jorine Arnouts, Senada Koljenović, Elise Daems, Karolien De Wael, Marc Peeters, Léon C. van Kempen, Greetje Vanhoutte, Karen Zwaenepoel, Timon Vandamme   +8 more
wiley   +1 more source

The i-Motif as a Molecular Target: More Than a Complementary DNA Secondary Structure. [PDF]

Pharmaceuticals (Basel), 2021
Brown SL, Kendrick S.
europepmc   +1 more source