POSoligo software for in vitro gene synthesis
Scientific ReportsOligonucleotide synthesis is vital for molecular experiments. Bioinformatics has been employed to create various algorithmic tools for the in vitro synthesis of nucleotides.
Yingying Tong +7 more
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Locked nucleic acid inhibits amplification of contaminating DNA in real-time PCR
BioTechniques, 2005 Locked nucleic acid (LNA®) is a modified DNA with increased binding affinity for complementary DNA sequences. Our strategy was to use this property of LNA to inhibit undesired PCR amplification (e.g., from contaminating genomic DNA) in a cDNA-based assay.
Lone Hummelshoj +3 more
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Data analysis of viral complementary DNA/RNA sequences for low-frequency intrahost-single nucleotide variants in COVID-19. [PDF]
Proc Natl Acad Sci U S A, 2022 Chandra S.
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Molecular Oncology, EarlyView.MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin +14 more
wiley +1 more source
Rescue of the first alphanucleorhabdovirus entirely from cloned complementary DNA: An efficient vector for systemic expression of foreign genes in maize and insect vectors. [PDF]
Mol Plant Pathol, 2023 Kanakala S +5 more
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Molecular Oncology, EarlyView.Epithelial–mesenchymal transition (EMT) and tumor‐infiltrating lymphocytes (TILs) are associated with early breast cancer response to neoadjuvant chemotherapy (NAC). This study evaluated EMT and TIL shifts, with immunofluorescence and RNA sequencing, at diagnosis and in residual tumors as potential biomarkers associated with treatment response.
Françoise Derouane +16 more
wiley +1 more source
The i-Motif as a Molecular Target: More Than a Complementary DNA Secondary Structure. [PDF]
Pharmaceuticals (Basel), 2021 Brown SL, Kendrick S.
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Adverse prognosis gene expression patterns in metastatic castration‐resistant prostate cancer
Molecular Oncology, EarlyView.We aggregated a cohort of 1012 mCRPC tissue samples from 769 patients and investigated the association of gene expression‐based pathways with clinical outcomes. Loss of AR signaling, high proliferation, and a glycolytic phenotype were independently prognostic for poor outcomes, and an adverse transcriptional feature score incorporating these pathways ...
Marina N. Sharifi +26 more
wiley +1 more source
Cationic copolymers that enhance wild-type-specific suppression in BNA-clamp PCR and preferentially increase the T m of fully matched complementary DNA and BNA strands. [PDF]
Biol Methods Protoc, 2022 Tachibana A +5 more
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COMPLEMENTARY STRUCTURE OF INTERACTING SITES AT THE ENDS OF LAMBDA DNA MOLECULES [PDF]
, 1965 A. D. Hershey, Elizabeth Burgi
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