Results 161 to 170 of about 2,891,675 (191)

Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor.

Science, 1987
Low-stringency hybridization with human glucocorticoid receptor (hGR) complementary DNA was used to isolate a new gene encoding a predicted 107-kilodalton polypeptide.
J. Arriza, J. Arriza, C. Weinberger, C. Weinberger, G. Cerelli, T. Glaser, B. Handelin, D. Housman, R. Evans   +8 more
semanticscholar   +1 more source

Sequence and expression of human estrogen receptor complementary DNA.

Science, 1986
The mechanism by which the estrogen receptor and other steroid hormone receptors regulate gene expression in eukaryotic cells is not well understood. In this study, a complementary DNA clone containing the entire translated portion of the messenger RNA ...
G. Greene, P. Gilna, M. Waterfield, A. Baker, Y. Hort, J. Shine   +5 more
semanticscholar   +1 more source

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.

Science, 1985
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce
G. Wong, JS Witek, P. Temple, KM Wilkens, AC Leary, DP Luxenberg, Jones Ss, E. Brown, Kay Rm, Orr Ec   +9 more
semanticscholar   +1 more source

Cloning of human androgen receptor complementary DNA and localization to the X chromosome.

Science, 1988
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors ...
D. Lubahn, D. R. Joseph, Patrick F. Sullivan, H. Willard, F. S. French, E. M. Wilson   +5 more
semanticscholar   +1 more source

Molecular cloning of human and rat complementary DNA encoding androgen receptors.

Science, 1988
Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA ...
Chawnshang Chang, J. Kokontis, S. Liao
semanticscholar   +1 more source

Cloned poliovirus complementary DNA is infectious in mammalian cells.

Science, 1981
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus.
V. Racaniello, D. Baltimore
semanticscholar   +1 more source

Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila.

Science, 1987
On the basis of electrophysiological analysis of Shaker mutants, the Shaker locus of Drosophila melanogaster has been proposed to encode a structural component of a voltage-dependent potassium channel, the A channel. Unlike sodium channels, acetylcholine
D. Papazian, T. Schwarz, B. Tempel, Y. Jan, L. Jan   +4 more
semanticscholar   +1 more source

Molecular cloning of the complementary DNA for human tumor necrosis factor.

Science, 1985
Tumor necrosis factor (TNF) is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF was purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and showed cytotoxic and ...
A. Wang, A. Creasey, M. Ladner, Leo S. Lin, J. Strickler, JN Van Arsdell, R. Yamamoto, D. Mark   +7 more
semanticscholar   +1 more source

Purification and complementary DNA cloning of a receptor for basic fibroblast growth factor.

Science, 1989
Basic fibroblast growth factor (bFGF) participates in many processes including early developmental events, angiogenesis, wound healing, and maintenance of neuronal cell viability.
Pauline E. Lee, Daniel E. Johnson, L. Cousens, V. Fried, L. Williams   +4 more
semanticscholar   +1 more source

Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).

Science, 1985
Complementary DNA (cDNA) clones encoding human macrophage-specific specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids.
E. Kawasaki, M. Ladner, A. Wang, JN Van Arsdell, M. Warren, M. Coyne, V. Schweickart, MT Lee, K. Wilson, A. Boosman   +9 more
semanticscholar   +1 more source