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Statistical evaluation of the coding capacity of complementary DNA strands [PDF]
Two independent methods are used to evaluate the protein-coding information content in different classes of DNA sequences. The first method allows to evaluate the statistical relevance of finding unidentified reading frames, longer than 100 codons, on both DNA strands of: a) 117 DNA sequences that code for 142 nuclear proteins; b) 39 stable RNA coding ...
Anna Tramontano+6 more
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Coronavirus (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified as a deadly pandemic. The genomic analysis of SARS-CoV-2 is performed using a reverse transcription-polymerase chain reaction (RT-PCR)
Dalal A. Aloraini+4 more
doaj +1 more source
The isolation of plasmids containing dna complementary to messenger rna for variant surface glycoproteins of Trypanosoma brucei [PDF]
We have isolated poly(A)+ RNA from four antigenic variants (117, 118, 121, 221) of one clone of Trypanosoma brucei. Translation of these poly(A)+ RNAs in a rabbit reticulocyte lysate gave rise to proteins that could be precipitated with antisera against ...
Jan H.J. Hoeijmakers+4 more
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MGB polyamide-oligonucleotide conjugates ON 1-4 with linked MGB polyamides at the 2-exocyclic amino group of a guanine base using aminoalkyl linkers were synthesized and evaluated in terms of binding affinity for complementary DNA containing the MGB ...
Kazuo Kamaike+13 more
doaj +1 more source
A Brief Review of Graphene-Based Biosensors Developed for Rapid Detection of COVID-19 Biomarkers
The prevalence of mutated species of COVID-19 antigens has provided a strong impetus for identifying a cost-effective, rapid and facile strategy for identifying the viral loads in public places.
Narendhar Chandrasekar+7 more
doaj +1 more source
Coding capacity of complementary DNA strands [PDF]
A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100 codons ("virtual genes") are ...
Casino A.+5 more
openaire +5 more sources
Cloning of human thyroglobulin complementary DNA [PDF]
Journal Article ; Research Support, Non-U.S.
Brocas, Huguette+3 more
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Mechanical separation of the complementary strands of DNA [PDF]
We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage λ DNA. The 3′ and 5′ extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10–15 pN. The separation force signal is
François Heslot+2 more
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Complementary DNA Libraries and Neurological Disease [PDF]
The DNA in our cell nuclei contains all the information necessary to direct our biological functions. The gene is 1 unit of such information that usually specifies the expression of 1 protein product. A detailed knowledge of each gene will enable us to understand disease at the molecular level.
Henry Furneaux, Benjamin Joseph
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Background Bivalent thrombin‐binding aptamers (TBAs) have great potential for the treatment of thrombosis because they exhibit high anticoagulant activity, and their complementary single‐stranded DNA (ssDNA) sequences work as an antidote.
Toru Yoshitomi+3 more
doaj +1 more source