Results 21 to 30 of about 2,891,675 (191)

Simplified detection of the hybridized DNA using a graphene field effect transistor

open access: yesScience and Technology of Advanced Materials, 2017
Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA).
Arun Kumar Manoharan   +3 more
doaj   +1 more source

Ion-Channel Genosensor for the Detection of Specific DNA Sequences Derived from Plum Pox Virus in Plant Extracts

open access: yesSensors, 2014
A DNA biosensor for detection of specific oligonucleotides sequences of Plum Pox Virus (PPV) in plant extracts and buffer is proposed. The working principles of a genosensor are based on the ion-channel mechanism. The NH2-ssDNA probe was deposited onto a
Kamila Malecka   +3 more
doaj   +1 more source

Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus

open access: yesSensors, 2013
A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution ...
Chee-Seng Toh, Jiajia Deng
doaj   +1 more source

Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA

open access: yesMolecules, 2022
The development of chemically modified oligonucleotides enabling robust, sequence-unrestricted recognition of complementary chromosomal DNA regions has been an aspirational goal for scientists for many decades.
Caroline P. Shepard   +3 more
doaj   +1 more source

Preparation of fluorescent DNA probe by solid-phase organic synthesis

open access: yeseXPRESS Polymer Letters, 2009
Fluorescent DNA probe based on fluorescence resonance energy transfer (FRET) was prepared by solid-phase organic synthesis when CdTe quantum dots (QDs) were as energy donors and Au nanoparticles (AuNPs) were as energy accepters.
doaj   +1 more source

Label-free and sensitive fluorescent detection of sequence-specific single-strand DNA based on S1 nuclease cleavage effects. [PDF]

open access: yesPLoS ONE, 2014
The ability to detect sequence-specific single-strand DNA (ssDNA) in complex, contaminant-ridden samples, using a fluorescent method directly without a DNA extraction and PCR step could simplify the detection of pathogens in the field and in the clinic ...
Zheng Guan   +7 more
doaj   +1 more source

Simulations Meet Experiment to Reveal New Insights into DNA Intrinsic Mechanics.

open access: yesPLoS Computational Biology, 2015
The accurate prediction of the structure and dynamics of DNA remains a major challenge in computational biology due to the dearth of precise experimental information on DNA free in solution and limitations in the DNA force-fields underpinning the ...
Akli Ben Imeddourene   +5 more
doaj   +1 more source

Non-homologous DNA end joining in normal and cancer cells and its dependence on break structures

open access: yesGenetics and Molecular Biology, 2010
DNA double-strand breaks (DSBs) are a serious threat to the cell, for if not or miss-repaired, they can lead to chromosomal aberration, mutation and cancer.
Tomasz Poplawski   +2 more
doaj  

Experimental validation of determinants of UV sensitivity using synthetic DNA

open access: yesJournal of Photochemistry and Photobiology, 2022
Treatment with ultraviolet (UV) light has been shown to be effective for disinfection of pathogens. For pathogenic risk management, it would be effective to estimate the UV sensitivity of unknown pathogenic microorganisms from their nucleotide sequences.
M. Otaki, Y. Higashino, Y. Yamada
doaj  

Cloning of complementary DNA encoding a functional human interleukin-8 receptor.

open access: yesScience, 1991
Interleukin-8 (IL-8) is an inflammatory cytokine that activates neutrophil chemotaxis, degranulation, and the respiratory burst. Neutrophils express receptors for IL-8 that are coupled to guanine nucleotide-binding proteins (G proteins); binding of IL-8 ...
P. Murphy, H. Tiffany
semanticscholar   +1 more source

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