Results 331 to 340 of about 3,270,422 (387)

A membrane-filter technique for the detection of complementary DNA.

Biochemical and Biophysical Research Communications, 1966
D. Denhardt
semanticscholar   +4 more sources

Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA

Science, 1989
Overlapping complementary DNA clones were isolated from epithelial cell libraries with a genomic DNA segment containing a portion of the putative cystic fibrosis (CF) locus, which is on chromosome 7.
J. Riordan
semanticscholar   +2 more sources

Complementary DNA-DNA hybridization in Drosophila

Journal of Molecular Evolution, 1992
We have performed DNA-DNA hybridization experiments among several species of Drosophila using the evolutionarily conserved portion of the genome representing sequences coding for amino acids of proteins. This was done by using as tracer, radioactively labeled complementary DNA that was reverse transcribed from adult mRNA.
Jennifer M. Gleason, Adalgisa Caccone, Jeffrey R. Powell   +2 more
openaire   +3 more sources

Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor.

Science, 1987
Low-stringency hybridization with human glucocorticoid receptor (hGR) complementary DNA was used to isolate a new gene encoding a predicted 107-kilodalton polypeptide.
J. Arriza, J. Arriza, C. Weinberger, C. Weinberger, G. Cerelli, T. Glaser, B. Handelin, D. Housman, R. Evans   +8 more
semanticscholar   +1 more source

Interaction of complementary RNA and DNA

Journal of Molecular Biology, 1964
Double-strand formation occurs with high molecular weight DNA embedded in agar on incubation with either messenger RNA or sheared, denatured DNA. Both the DNA in the agar and the DNA fragments must be previously denatured for reaction to occur. The fraction of the total RNA in E. coli represented by the messenger type is about 1·3%.
B.J. McCarthy, E.T. Bolton
openaire   +3 more sources

A Drosophila Complementary DNA Resource

Science, 2000
Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. We describe a general strategy for producing such collections and its implementation, which so far has generated a set of cDNAs corresponding to over 40%
Ling Hong, Peter Brokstein, Damon A. Harvey, Damon A. Harvey, Martha Evans-Holm, Mark Stapleton, Erwin Frise, Gerald M. Rubin, Gerald M. Rubin   +8 more
openaire   +3 more sources

Sequence and expression of human estrogen receptor complementary DNA.

Science, 1986
The mechanism by which the estrogen receptor and other steroid hormone receptors regulate gene expression in eukaryotic cells is not well understood. In this study, a complementary DNA clone containing the entire translated portion of the messenger RNA ...
G. Greene, P. Gilna, M. Waterfield, A. Baker, Y. Hort, J. Shine   +5 more
semanticscholar   +1 more source

Strategies for constructing complementary DNA for cloning

Journal of Theoretical Biology, 1982
Abstract We have examined alternative approaches to existing methods for synthesizing complementary DNA suitable for molecular cloning. One model of construction is presented in which ribonucleotides are added to the 3′ end of complementary DNA prior to synthesis of the second DNA strand.
Jim Gaubatz, Gary V. Paddock
openaire   +3 more sources

Complementary strands of CELO virus DNA

Journal of Virology, 1975
When alkali-denatured DNA from CELO virus (an avian adenovirus) was annealed for 15 min at 37 C in 0.1 M NaCl, 70% of the molecules formed single-stranded circles. This is probably due to base pairing of complementary sequences not more than 110 nucleotides long at the ends of the single strands and implies an inverted terminal repetition in the duplex
A J Bellett, A J Robinson
openaire   +3 more sources

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.

Science, 1985
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce
G. Wong, JS Witek, P. Temple, KM Wilkens, AC Leary, DP Luxenberg, Jones Ss, E. Brown, Kay Rm, Orr Ec   +9 more
semanticscholar   +1 more source