Results 341 to 350 of about 3,270,422 (387)

Cloning of human androgen receptor complementary DNA and localization to the X chromosome.

Science, 1988
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors ...
D. Lubahn, D. R. Joseph, Patrick F. Sullivan, H. Willard, F. S. French, E. M. Wilson   +5 more
semanticscholar   +1 more source

Complementary DNA Libraries: An Overview

Molecular Biotechnology, 2003
The generation of complete and full-length cDNA libraries for potential functional assays of specific gene sequences is essential for most molecules in biotechnology and biomedical research. The field of cDNA library generation has changed rapidly in the past 10 yr.
openaire   +5 more sources

Development of a murine hematopoietic progenitor complementary DNA microarray using a subtracted complementary DNA library

Blood, 2002
AbstractWith the goal of creating a resource for in-depth study of myelopoiesis, we have executed a 2-pronged strategy to obtain a complementary DNA (cDNA) clone set enriched in hematopoietic genes. One aspect is a library subtraction to enrich for underrepresented transcripts present at early stages of hematopoiesis.
Olga Mironenko, Sharon Lin, Diane S. Krause, Steven M. Johnson, Nancy Berliner, Tupur Husain, Noel Maun, David Tuck, Xianyong Ma, Hui Peng, Archibald S. Perkins   +10 more
openaire   +3 more sources

Molecular cloning of human and rat complementary DNA encoding androgen receptors.

Science, 1988
Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA ...
Chawnshang Chang, J. Kokontis, S. Liao
semanticscholar   +1 more source

“DNA-Dressed NAnopore” for complementary sequence detection

Biosensors and Bioelectronics, 2011
Single molecule electrical sensing with nanopores is a rapidly developing field with potential revolutionary effects on bioanalytics and diagnostics. The recent success of this technology is in the simplicity of its working principle, which exploits the conductance modulations induced by the electrophoretic translocation of molecules through a ...
MUSSI, VALENTINA, P. Fanzio, REPETTO, LUCA, FIRPO, GIUSEPPE, S. Stigliani, G. P. Tonini, VALBUSA, UGO   +6 more
openaire   +3 more sources

Cloned poliovirus complementary DNA is infectious in mammalian cells.

Science, 1981
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus.
V. Racaniello, D. Baltimore
semanticscholar   +1 more source

Cloning of genomic and complementary DNA from Shaker, a putative potassium channel gene from Drosophila.

Science, 1987
On the basis of electrophysiological analysis of Shaker mutants, the Shaker locus of Drosophila melanogaster has been proposed to encode a structural component of a voltage-dependent potassium channel, the A channel. Unlike sodium channels, acetylcholine
D. Papazian, T. Schwarz, B. Tempel, Y. Jan, L. Jan   +4 more
semanticscholar   +1 more source

Patents on Random Complementary DNA Fragments?

Science, 1992
The proposal by the National Institutes of Health (NIH) to patent products resulting merely from sequencing the human genome is a mistake: at worst, it is wrong in patent law; at best, it relies on deficiencies in law concerning what is "useful" as a requirement for patents.
openaire   +2 more sources

Molecular cloning of the complementary DNA for human tumor necrosis factor.

Science, 1985
Tumor necrosis factor (TNF) is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF was purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and showed cytotoxic and ...
A. Wang, A. Creasey, M. Ladner, Leo S. Lin, J. Strickler, JN Van Arsdell, R. Yamamoto, D. Mark   +7 more
semanticscholar   +1 more source

The binding of polyribonucleotides to the complementary strands of mitochondrial DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
Abstract We have purified the complementary strands (H and L) of rat mtDNA by equilibrium centrifugation in alkaline CsCl and studied their interaction with polyribonucleotides in neutral CsCl. H, the strand with the higher density in alkaline CsCl, was shifted to a higher density by poly(C) (20 mg/cm3), slightly shifted by alkali-degraded poly(U) (7
Piet Borst, G.J.C.M. Ruttenberg
openaire   +3 more sources