Results 351 to 360 of about 3,270,422 (387)
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Purification and complementary DNA cloning of a receptor for basic fibroblast growth factor.

Science, 1989
Basic fibroblast growth factor (bFGF) participates in many processes including early developmental events, angiogenesis, wound healing, and maintenance of neuronal cell viability.
Pauline E. Lee   +4 more
semanticscholar   +1 more source

Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1).

Science, 1985
Complementary DNA (cDNA) clones encoding human macrophage-specific specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids.
E. Kawasaki   +9 more
semanticscholar   +1 more source

Gene specific priming of complementary DNA synthesis

Molecular Biology Reports, 1976
DNA, complementary to chicken globin mRNA was synthesized using either Avian Myeloblastosis virus reverse transcriptase, or E. coli DNA polymerase I. Transcriptase cDNA sediments at 9 S on sucrose gradients, and is 620 nucleotides in length, representing a complete copy of globin mRNA template. In contrast, Polymerase I cDNA sediments at 4 S, is 100 to
J. R. E. Wells, R. J. Crawford
openaire   +3 more sources

Molecular cloning of complementary DNA encoding the avian receptor for vitamin D.

Science, 1987
Vitamin D3 receptors are intracellular proteins that mediate the nuclear action of the active metabolite 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. Two receptor-specific monoclonal antibodies were used to recover the complementary DNA (cDNA) of this ...
D. McDonnell   +4 more
semanticscholar   +1 more source

Nucleotide Sequence of Human Preproinsulin Complementary DNA

Science, 1980
Recombinant bacterial plasmids that contain DNA complementary to human preproinsulin messenger RNA have been constructed. One clone contains the entire preproinsulin coding region, as well as the 3′ untranslated region of the messenger RNA and eight nucleotides of the 5′ untranslated region.
David V. Goeddel   +3 more
openaire   +3 more sources

Complementary DNA Synthesis in Situ: Methods and Applications

1992
In situ transcription is the synthesis of cDNA within cells. This chapter has illustrated some of the application of IST to the study of gene expression in complex cell environments. While the importance of transcription in modulating cellular activity has been long appreciated, the role of translational control mechanisms in regulating central nervous
James Eberwine   +4 more
openaire   +3 more sources

Cloning and characterization of canine thyroglobulin complementary DNA

Domestic Animal Endocrinology, 2007
Canine thyroglobulin (cTg) is one of the thyroid autoantigens associated with hypothyroidism caused by autoimmune thyroiditis in dog. To identify canine-specific areas in cTg, we cloned, by reverse transcriptase PCR, and sequenced the complete cDNA of cTg.
Yuji Uzuka   +5 more
openaire   +3 more sources

The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA.

Biochemistry, 1976
The synthesis of a complementary DNA copy (cDNA) of hen ovalbumin mRNA using AMV RNA-directed DNA polymerase was studied under different conditions of salt, deoxyribonucleotide concentrations, temperature, and time. It was observed that in the absence of
J. Monahan   +4 more
semanticscholar   +1 more source

Rapid Amplification of Complementary DNA Ends for Generation of Full-Length Complementary DNAs: Thermal RACE

1993
Publisher Summary This chapter discusses about the rapid amplification of complementary DNA (cDNA) ends for the generation of full length cDNAs. The generation of a full-length cDNA represents the most challenging part of a cloning project, particularly with respect to obtaining the 5′ end of the cDNA.
openaire   +4 more sources

Nucleotide sequence of a complementary DNA for human ST2

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
Human ST2 cDNA, a homologue of murine ST2 that is only expressed in growth-stimulated BALB/c-3T3 cells and a member of the primary response gene family induced by growth factors, was isolated from the cDNA library of an activated human helper T cell line, 5C10. Human ST2 has 67.6% identity in a 327 amino acid overlap to murine ST2.
Toshihiko Tsukamoto   +5 more
openaire   +3 more sources

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