Results 111 to 120 of about 105,174 (312)
Research on Weak Beam-strong Column Design Method of Steel-concrete Composite Frame Structures
工程科学与技术, 2012 :In order to solve the problem that for general composite frame consisting of concrete-filled steel tubular (CFT) columns and steel-concrete composite beams (CB), the method to achieve strong column-weak beam behavior is not available in China code, GB ...
doaj
Results in EngineeringThe goal of this paper is to present the behavior and performance of a fully encased composite column subjected to horizontal cyclic load using the Finite Element Simulation.
Regasa yadeta Sembeta +3 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Composite column - Calculation examples [PDF]
, 2010 Composite columns are often used in structures due to the ease and speed of erection, and high performance in fire situation. Concrete filled tubes are steel tubes that are in site filled with reinforced concrete.
Bzdawka, Karol
core
Experimental Investigation on Steel-Plate-Masonry Composite Column Compressive Behavior
, 2011 The steel-plate-masonry composite structure is an innovative type of structural scheme popular in masonry structures with load-bearing walls removed for a large space.
Deng Hu Jing +2 more
core +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Optimization of a Composite Wing Subject to Multi Constraints [PDF]
, 2013 In this thesis, an investigation has been carried out into a minimum weight optimization analysis of a composite wing with multi design constraints under both static and dynamic loadings. The study includes the influence of a morphing leading edge on the
Fu, Qiang
core
, 2011 Based on the model tests and the results of theoretical research, considered elasto-plasticity of the soil and highly nonlinear behavior of contact with columns and soil, more reasonable numerical model on multi-element composite foundation of concrete ...
Xian Zhi Wang
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source