Results 121 to 130 of about 260,987 (350)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Hardware Trojan Detection in Open-Source Hardware Designs Using Machine Learning
IEEE AccessThe globalization of the hardware supply chain reduces costs but increases security challenges with the potential insertion of hardware trojans by third parties.
Victor Takashi Hayashi +1 more
doaj +1 more source
Revisiting the Relationship between Software Architecture and Requirements : the case of Dynamically Adaptive Systems [PDF]
, 2009 This paper revisits the relationship between software architecture and requirements focusing on the case of selfadaptive systems. The authors present their view of the state-of-the-art, including their own work, on both areas and their contribution ...
Sawyer, Peter, Grace, P., Bencomo, Nelly
core
An active-architecture approach to COTS integration
, 2011 Commercial off-the-shelf (COTS) software products are increasingly used as standard components within integrated information systems. This creates challenges since both their developers and source code are not usually available, and the ongoing ...
Balasubramaniam, Dharini +8 more
core +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
How accurate is low-cost comfort monitoring? Validating the ComfortCube [PDF]
E3S Web of Conferences. The reliable measurement of indoor climate parameters is a fundamental prerequisite for evaluating indoor climate and, consequently, user comfort. While professional measurement systems offer high precision, they are often associated with high costs ...
Mill Senta +4 more
doaj +1 more source
A reflective architecture to support dynamic software evolution [PDF]
, 2002 This thesis presents work which is concerned with the run-time evolution of component-based software systems. In particular, the main result of the research presented here is a framework which is used to model and control the architecture of a software ...
Rank, Stephen
core
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
YIPFα1A expression is regulated by multilayered molecular mechanisms
FEBS Open Bio, EarlyView.YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji +2 more
wiley +1 more source