Results 61 to 70 of about 35,400 (213)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
The Graphic Descriptor Ontology: An ontology for semantic annotation of graphics and its application to graphic libraries of anatomical standards. [PDF]
PLoS OneClarkson MD, Perry NC, Detwiler LT.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
An empirical exploration of the diversified R ecosystem. [PDF]
PLoS OneHuang TY, Lou Z.
europepmc +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Elements and roadmap for interactive molecular graphics and modeling "in the Holodeck". [PDF]
Protein SciMulholland AJ, Abriata LA.
europepmc +1 more source
COGGRAPH: Building bridges between cognitive science and computer graphics. [PDF]
In recent years, the field of computer graphics has achieved its longstanding dream of photorealism: modern graphics algorithms produce images that are indistinguishable from reality. Much like art at the advent of photography, then, computer graphics is now turning its gaze to the beholder: researchers are increasingly looking to cognitive science to ...
Chandra, Kartik +7 more
openaire +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Sensing Techniques in Virtual Reality for Human Interaction: A Bibliometric Analysis. [PDF]
Sensors (Basel)Del Bosque A +2 more
europepmc +1 more source