Results 101 to 110 of about 128,795 (262)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

CONCEPTUALIZING THE MULTIPLICITY OF INTEGRATION

Information Technology and Control, 2007
Integration of complex socio-technical networks is the goal and challenge for today’s organization. Surprisingly, such a central term ‘integration’ is highly ambiguous and has multiply meanings. In this paper we expose the ambiguity of the term ‘integration’ and propose an interdisciplinary analytical perspective how integration acti-vities can be ...
openaire   +1 more source

A Conceptual Framework for Multi-Way Integration (MUWI) With a Focus on Residents’ Multiple Identities and Intersectionalities

Dve Domovini
Conceptual frameworks on integration have moved from one to two-way integration, focusing on all residents in integration processes. This paper advocates a conceptual framework for “multi-way integration” (MUWI), which focuses explicitly on residents ...
Claudia Schneider
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Lost in "Conceptual Space": Metaphors of Conceptual Integration

Metaphor and Symbol, 2004
Conceptual integration theory (Fauconnier & Turner, 1998, 2002) is re-examined in the light of recent criticisms (e.g., Gibbs, 2000, 2001). It is argued that the assumption of four independent "cognitive spaces" enters the model primarily as an entailment of the "space," "blending," and "construction" metaphors, leads to unnecessary ambiguity, and ...
openaire   +3 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Living labs as orchestrators in the regional innovation ecosystem: a conceptual framework

Journal of Responsible Innovation
This research explores the conceptual integration of Living Labs (LLs) into the Regional Innovation Ecosystem (RIE) to understand their potential as facilitators of Responsible Innovation.
Janin Fauth, Kevin De Moortel, Dimitri Schuurman   +2 more
doaj   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Analysis of students’ conceptual change in learning Newton’s third law with an integrated framework of model analysis and knowledge integration

Physical Review Physics Education Research
The purpose of this study is to examine how students may hold coexisting and competing conceptual ideas and how students’ uses of such ideas may evolve during the process of conceptual change by combining two complementary methods including model ...
Yi Ding, Guangtian Zhu, Qiuxin Bian, Lei Bao   +3 more
doaj   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source