Results 31 to 40 of about 180,806 (263)

Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope

Frontiers in Neuroanatomy, 2019
Human inducible pluripotent stem cells (hiPSCs) hold a large potential for disease modeling. hiPSC-derived human astrocyte and neuronal cultures permit investigations of neural signaling pathways with subcellular resolution.
Irina Rakotoson, Irina Rakotoson, Irina Rakotoson, Irina Rakotoson, Brigitte Delhomme, Brigitte Delhomme, Brigitte Delhomme, Philippe Djian, Philippe Djian, Philippe Djian, Andreas Deeg, Maia Brunstein, Maia Brunstein, Maia Brunstein, Christian Seebacher, Rainer Uhl, Clément Ricard, Clément Ricard, Clément Ricard, Martin Oheim, Martin Oheim, Martin Oheim   +21 more
doaj   +1 more source

Effects of Tea Polyphenols and Theaflavins on Three Oral Cariogenic Bacteria

Molecules, 2023
In order to investigate the antibacterial mechanism of tea polyphenols and theaflavins against oral cariogenic bacteria, the pH value of the culture medium, the number of bacteria adhering to the smooth glass tube wall, and the electrical conductivity ...
Xia Cui, Lei Xu, Kezhen Qi, Hai Lan
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Use of Confocal Laser Scanning Microscope in Cytochemistry.

ACTA HISTOCHEMICA ET CYTOCHEMICA, 1994
The applications of a confocal laser scanning microscope for imaging fast dynamic changes of the intracellular calcium ion concentration ([Ca2+]i) in the living cell are described. Confocal images enable us to determine the cell-to-cell junction between clustered heart muscle cells of guinea pig and reveal that the high [Ca2+]i can evoke calcium ...
TAKAMATSU, TETSURO, FUJITA, SETSUYA
openaire   +2 more sources

An in vitro Comparative evaluation of the sealing ability of five different root-end filling materials under confocal laser microscopy

Contemporary Clinical Dentistry, 2020
Aim: The purpose of this in-vitro study was to compare and evaluate the best sealing ability of five different root end filling materials i.e. Silver Amalgam, RMGIC, Cermet Cement, MTA Angelus and Biodentine using ConFocal Laser Scanning Microscope ...
Fatinder Jeet Singh, Lovejeet Ahuja, Gurpreet Kakkar, Ashish Kakkar, Abhinav Garg, Akshita Mahajan   +5 more
doaj   +1 more source

Isolation, cryo-laser scanning confocal microscope imaging and cryo-FIB milling of mouse glutamatergic synaptosomes.

PLoS ONE, 2022
Ionotropic glutamate receptors (iGluRs) at postsynaptic terminals mediate the majority of fast excitatory neurotransmission in response to release of glutamate from the presynaptic terminal.
Prerana Gogoi, Momoko Shiozaki, Eric Gouaux   +2 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Super-resolution for 2.5D height data of microstructured surfaces using the vdsr network [PDF]

EPJ Web of Conferences, 2020
In this work super-resolution imaging is used to enhance 2.5D height data of thermal sprayed Al2O3 ceramics with stochastically microstructured surfaces. The data is obtained by means of a confocal laser scanning microscope.
Siemens Stefan, Kästner Markus, Reithmeier Eduard   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source