Results 31 to 40 of about 2,536,050 (286)
FEBS Open Bio, 2021 Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures.
Simon Hennig, Dietmar J. Manstein
doaj +1 more source
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells [PDF]
FEBS Open BioMicroscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Hink M.
europepmc +2 more sources
Microscopic Spectrophotometry Using Confocal Laser Scanning Microscope
BUNSEKI KAGAKU, 2008 共焦点レーザー走査型顕微鏡（CLSM）を用いた顕微吸光度分析の精度及び適応範囲などを検証するために，粒径が50及び150 μmの2種類の球状シリカゲルにジヒドロオキソ（テトラフェニルポルフィリナト）アンチモン臭化物錯体（1）を0.70～3.59 mmol dm－3のモル濃度（C1m）で吸着させてマイクロビーズ（2）を調製した．CLSMでの試料の測定領域は，対物レンズの倍率及びピンホールの直径又はファイバーコアの直径によって決められる直径が2.14 μmの円形内であることが分かった．更に分析範囲，測定範囲などの分析条件を検討した結果，4.9～350 μmの球状試料について測定波長が488～775 nmで分析可能であることが分かった．そこで ...
Matsumoto, Tomoko +4 more
openaire +2 more sources
Differences of size and shape of active and inactive X-chromosome domains in human amniotic fluid cell nuclei [PDF]
, 1993 It is a widely held belief that the inactive X-chromosome (Xi) in female cell nuclei is strongly condensed as compared to the largely decondensed active X-chromosome (Xa).
Cremer, Thomas +11 more
core +1 more source
Enhanced scanning control in a confocal scanning laser microscope [PDF]
Scanning, 1996 AbstractA fast and flexible scanning unit, allowing scanning rates of more than 1 kHz over regions identified in a specimen, has been developed and evaluated. This scanning unit replaces the original scanning unit in the Phoibos confocal scanning laser microscope and features full backward compatibility, while at the same time allowing fast and ...
openaire +2 more sources
BMC Oral HealthBackground An endodontic sealer forms an interface between gutta-percha and root canal walls. Ideally, the sealer layer should be void-free, thin, uniform, well-adapted to the dentinal walls, and exhibit good tubular penetration, which contributes to a ...
Avishikta Banerjee +4 more
doaj +1 more source
BioTechniques, 2015 Multispectral karyotyping analyzes all chromosomes in a single cell by labeling them with chromosome-specific probes conjugated to unique combinations of fluorophores.
Tamara A. Potapova +8 more
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Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells
FEBS Letters, EarlyView.DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park +8 more
wiley +1 more source
The Laser-Scanning Confocal Microscope in Biomedical Research
Experimental Biology and Medicine, 1991 The laser-scanning confocal microscope (LSCM) produces improved light microscope images of both fixed and living cells and tissues. Moreover, the serial optical-sectioning power of the LSCM has made three dimensional reconstruction of light microscope images a practical option.
openaire +2 more sources
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source