Results 51 to 60 of about 2,536,050 (286)

A confocal beam scanning white-light microscope

, 1991
We report on a confocal beam scanning microscope utilizing a continuous Xe short-arc lamp operating in the visible spectrum with unprecedented radiance.
E. Smolka, R. W. Wijnaendts Resandt, Resandt, R. W. Wijnaendts, Neiger, M., S. Hell, M. Neiger, S. Witting, Hunklinger, S., Smolka, E., Schickfus, M., Hell, S., Witting, S., S. Hunklinger, M. Schickfus   +13 more
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

The development of a time-resolved confocal microscope for single molecule detection of luminescent polymers [PDF]

, 2007
This report details the construction and testing of a confocal microscope for use in single molecule fluorescence spectroscopy. With the prospect of making time-resolved lifetime and spectral measurements of π-conjugated polymers in the condensed phase ...
Mc Auley, Frances
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Antinuclear human autoantibodies as markers in Nicotiana tabacum pollen tubes

Acta Societatis Botanicorum Poloniae, 2014
In the present paper we report on the use of antinuclear human autoantibodies as specific markers in Nicotiana tabacum pollen tubes. The antibodies have been tested by fluorescence techniques using a confocal laser scanning microscope. All the antibodies
C. Poggialini, G. Morozzi, R. Marcolongo, R. Vignani, M. Cresti, A. Tiezzi   +5 more
doaj   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Design of a quantum diamond microscope with efficient scanning confocal readout

Academia Quantum
In this paper we introduce the light-sheet confocal quantum diamond microscope (LC-QDM) for widefield 3D quantum sensing with efficient confocal readout.
Daniel G. Ang, Jiashen Tang, Ronald L. Walsworth   +2 more
doaj   +1 more source

Plasmon resonance and the imaging of metal-impregnated neurons with the laser scanning confocal microscope

eLife, 2015
The staining of neurons with silver began in the 1800s, but until now the great resolving power of the laser scanning confocal microscope has not been utilized to capture the in-focus and three-dimensional cytoarchitecture of metal-impregnated cells ...
Karen J Thompson, Cynthia M Harley, Grant M Barthel, Mark A Sanders, Karen A Mesce   +4 more
doaj   +1 more source

Microanatomy of the Rat Diaphragm: A Scanning Electron and Confocal Laser Scanning Microscopic Study.

Archives of Histology and Cytology, 1993
The present study demonstrated the three-dimensional microstructure of the rat diaphragm by scanning electron microscopy (SEM) of either intact or alkali-treated tissues, enzyme-histochemistry, and confocal laser scanning microscopy (LSM). The peritoneal and pleural surfaces of the diaphragm were covered with mesothelial cells studded with microvilli ...
Ohtani, Yuko, Ohtani, Osamu, Nakatani, Toshio   +2 more
openaire   +3 more sources