Results 131 to 140 of about 31,204 (291)

Parametric Blind Deconvolution for Confocal Laser Scanning Microscopy (CLSM)-Proof of Concept

, 2008
We propose a method for the iterative restoration of fluorescence Confocal Laser Scanning Microscope (CLSM) images with parametric estimation of the acquisition system's Point Spread Function (PSF). The CLSM is an optical fluorescence microscope that scans a specimen in 3D and uses a pinhole to reject most of the out-of-focus light.
Pankajakshan, Praveen, Blanc-Féraud, Laure, Zhang, B., Kam, Zvi, Olivo-Marin, Jean-Christophe, Zerubia, Josiane   +5 more
openaire   +1 more source

Imaging hyphal growth of Physisporinus vitreus in Norway spruce wood by means of confocal laser scanning microscopy (CLSM)

, 2017
Light microscopy and electron microscopy are the most common methods for analyzing wood-decay fungi. However, the 3D visualization and quantification of the filamentous structure of fungi in wood is difficult to realize by means of these traditional ...
Schubert, Mark, Fuhr, Matthias J., Stührk, Chris, Schwarze, Francis W.M.R.   +3 more
core  

CD44‐Targeting Hydroxyapatite Nanoparticles (HAP) Induce Mitochondrial Dysfunction‐Driven PANoptosis and Immunogenic Cell Death (ICD) via Ca Overload in Colorectal Cancer

Advanced Science, EarlyView.
ABSTRACT Colorectal cancer (CRC) remains therapeutically challenging due to high metastasis, recurrence, and immunotherapy resistance driven by tumor microenvironment‐mediated immune evasion. Immunogenic cell death (ICD) offers a promising strategy to reshape the immune microenvironment, yet existing ICD inducers suffer from poor targeting efficiency ...
Yao Xiao, Yuxuan Yang, Haosen Qiu, Jia Yang, Hao Liang, Zhaojun Jiang, Xiao Tong, Yongying Li, Qunfeng Huang, Jiaming Wu, Tian Lin, Jiang Yu, Min Liang   +12 more
wiley   +1 more source

Quantitative single-molecule imaging by confocal laser scanning microscopy

A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables ...
Terenius, Lars, Ming, Yu, Vukojevic, Vladana, Johansson, Bjoern, Rigler, Rudolf, Heidkamp, Marcus   +5 more
core   +1 more source

Diagnostic Applicability of In Vivo Confocal Laser Scanning Microscopy in Melanocytic Skin Tumors

, 2005
In vivo confocal laser scanning microscopy (CLSM) represents a novel imaging tool that allows the examination of skin morphology in real time at a resolution equal to that of conventional microscopes. The aim of the study was to test the applicability of
Gerger, Armin, Kerl, Helmut, Smolle, Josef, Massone, Cesare, Steiger, Karin, Koller, Silvia, Richtig, Erika, Kern, Thomas   +7 more
core   +1 more source

Dual‐Stage Microneedle Patch With Lipid Droplet‐Mediated ‘Capture‐Kill’ and Sustained Release for Enhanced Wound Healing

Advanced Science, EarlyView.
We report a lipid‐droplet‐engineered, gallium‐loaded sonodynamic therapeutic platform (LDTG) incorporated into a bilayer microneedle (MN) patch co‐delivering all‐trans retinoic acid (ATRA), termed LDTG A‐MNs, for synergistic antibacterial therapy and accelerated wound repair.
Peng Jiang, Meijun Jiang, Jiapeng Lei, Wen Zhang, Huiyuan Yang, Xueyang Wang, Siyu Zhang, Qiongfang Ruan, Shuhua Liu, Wei Li   +9 more
wiley   +1 more source

Lighting up Macrophage Reprogramming Assists Immunosuppressive Niche Modulation in Primary Tumors and Tumor‐Draining Lymph Nodes of Breast Cancer

Advanced Science, EarlyView.
This study develops an intelligent NIR‐II‐responsive nanoplatform to combat immunosuppression in breast cancer. It enables real‐time visualization of macrophage activity via “off‐on” fluorescence while simultaneously reprogramming macrophages through the CSF‐1R and CD47‐SIRPα pathways.
Yingbo Li, Di Chang, Jie Yang, Ying Bai, Min Chen, Daoshuang Li, Zuoyu Xu, Jiaxin Yuan, Xiaoxuan Xu, Zhiqi Zhang, Yize Li, Zixin Chen, Jinbing Xie, Zebin Xiao, Shenghong Ju   +14 more
wiley   +1 more source

Confocal laser scanning microscopy of calcium dynamics in living cells

Confocal laser scanning microscopy (CLSM) is widely used to monitor intracellular calcium levels in living cells loaded with calcium-sensitive fluorophores.
Stricker SA, Whitaker M
core  

Intracellular Trafficking of Carbon Nanotubes by Confocal Laser Scanning Microscopy

, 2007
A simple methodology to study the interactions between ammonium- functionalized single-walled carbon nanotubes (SWNT-NH3+), free of fluorescent labels, with mammalian cells was reported.
Bianco, Alberto, D. GATHERCOLE, Kostarelos, Kostas, Gathercole, David, L. LACERDA, A. BIANCO, G. PASTORIN, Buddle, Joanna, Lacerda, Lara, PRATO, MAURIZIO, J. BUDDLE, K. KOSTARELOS, Pastorin, Giorgia   +12 more
core   +1 more source

A Piezo‐Mimetic Ionic Hydrogel Harnessing Joint Motion for Cartilage Repair

Advanced Science, EarlyView.
A piezo‐mimetic ionic hydrogel converts mechanical deformation into bioactive ionic currents via stress‐induced asymmetric ion migration. Coupled with sustained Mg2+ release, this mechano‐electrochemical platform bridges mechanical loading and cellular signaling, enabling load‐adaptive stimulation and enhanced cartilage regeneration. ABSTRACT Articular
Chenyuan Gao, Xinyu Wang, Zhifeng Wu, Xinya Zhang, Xiao Geng, Ti Zhang, Yue Wang, Hongwei Xia, Jun Zhang, Xianbo Qiu, Cuiru Sun, Yingjie Yu, Wenli Dai, Hua Tian, Qing Cai   +14 more
wiley   +1 more source