Results 111 to 120 of about 2,617,420 (275)

Microbial Biofilm Detection and Differentiation by Dual Staining Using Maneval’s Stain

open access: yesBio-Protocol
Microbial biofilms are structured communities of microorganisms embedded in a self-produced extracellular matrix, adhering to surfaces. These biofilms enhance bacterial resistance to antibiotics, immune responses, and environmental stress.
B Nirmala, Balram Omar
doaj   +1 more source

Versatile Co‐Engineering of Immunogenic Cell Death‐Primed Dead Tumor Cell Bodies With Tumor‐Activated MnO2 for cGAS‐STING‐Potentiated Personalized Immunotherapy

open access: yesAdvanced Science, EarlyView.
DTBEI@CS@MnO2, fabricated via modular electrostatic co‐engineering and photothermal/photodynamic immunogenic cell death, specifically responds to the acidic and GSH‐rich tumor microenvironment. This triggers the release of DTBEI that provides tumor antigens, DNA, and DAMPs, as well as the generation of Mn2+, thereby synergistically potentiating cGAS ...
Bolun Xu   +6 more
wiley   +1 more source

Dual‐Responsive Hydrogels Integrate Bioelectrical Stimulation and Piezo‐Photocatalysis for Regenerative Treatment of Infected Wounds

open access: yesAdvanced Science, EarlyView.
A wireless, dual‐responsive hydrogel (PVA/(PVDF‐TrFE)/BiOClBr@PDA) is developed to promote efficient regeneration of infected wounds by integrating piezoelectric stimulation with piezo‐photocatalytic pathways. Under simultaneous light and ultrasonic stimulation, the hydrogel generates therapeutic electrical cues and reactive oxygen species, disrupting ...
Baoying Dai   +15 more
wiley   +1 more source

Analysis of polymer grafted inside the porous hydrogel using confocal laser scanning microscopy

open access: yeseXPRESS Polymer Letters, 2007
Graft polymerization of glycidyl methacrylate onto the pore surface of polyacrylamide macroporous gel was implemented in DMSO-aqueous solution using diperiodatocuprate(III) complexes as an initiator. The grafting densities up to 410% were achieved.
doaj   +1 more source

Parametric Blind Deconvolution for Confocal Laser Scanning Microscopy (CLSM)-Proof of Concept

open access: yes, 2008
We propose a method for the iterative restoration of fluorescence Confocal Laser Scanning Microscope (CLSM) images with parametric estimation of the acquisition system's Point Spread Function (PSF). The CLSM is an optical fluorescence microscope that scans a specimen in 3D and uses a pinhole to reject most of the out-of-focus light.
Pankajakshan, Praveen   +5 more
openaire   +1 more source

Confocal laser scanning microscopy to estimate nanoparticles’ human skin penetration in vitro

open access: yes, 2017
Ying Zou,1,2,* Anna Celli,2,3,* Hanjiang Zhu,2,* Akram Elmahdy,2 Yachao Cao,2 Xiaoying Hui,2 Howard Maibach2 1Skin & Cosmetic Research Department, Shanghai Skin Disease Hospital, Shanghai, People’s Republic of China; 2Department of Dermatology,
Zou Y   +6 more
core  

The development of a time-resolved confocal microscope for single molecule detection of luminescent polymers [PDF]

open access: yes, 2007
This report details the construction and testing of a confocal microscope for use in single molecule fluorescence spectroscopy. With the prospect of making time-resolved lifetime and spectral measurements of π-conjugated polymers in the condensed phase ...
Mc Auley, Frances
core  

Tailored Covalent Organic Frameworks Enable the Encapsulation of Living Cells to Produce Rare Ginsenoside

open access: yesAdvanced Science, EarlyView.
This work reports the in situ fabrication of Escherichia coli@covalent organic frameworks (E. coli@COFs) in phosphate‐buffered saline. The COFs form an exoskeleton on E. coli, protecting intracellular β‐glucosidase (Ssbgl) while enhancing mass transfer.
Fengmei Liu   +6 more
wiley   +1 more source

Laser-based fluorescence microscopy

open access: yes, 2002
[Introduction]:Many microbiologists will be familiar with transmission fluorescence microscopy as well the improved epifluorescence microscopy. However, microbiologists are generally less familiar with confocal laser scanning microscopy (CLSM), despite ...
Learmonth, Robert P.
core   +1 more source

Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy

open access: yesFrontiers in Dentistry, 2009
Objective: Confocal laser scanning microscopy (CLSM) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of CLSM for the biologist is its ability to provide optical sections
KA. Al-Salihi   +1 more
doaj  

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