Results 161 to 170 of about 37,668 (284)

Systematic Investigation of Impact of Antifoam and Extracellular Vesicles on Fouling of Hollow Fiber Filters in Intensified Perfusion Processes Highlights the Key Impact of Antifoam

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Ultra‐high cell density perfusion cultures are becoming increasingly attractive for biologics manufacturing due to their ability to boost productivity and offer a flexible manufacturing footprint. However, these intensified perfusion processes pose challenges, particularly concerning the performance of hollow fiber filters used as cell ...
Sri Ranganayaki Madabhushi, Qianying Zuo, Carol Zhou, Chelsea Phuangthong, Nathaniel Ruppert, Tiffany Tang, Francisco Pelaez   +6 more
wiley   +1 more source

Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations

Biotechnology Progress, EarlyView.
Abstract The possibility to produce recombinant adeno‐associated virus (rAAV) by adherent HEK293T cells was studied in a stirred tank bioreactor (STR) culture of cell aggregates. A proof‐of‐concept of rAAV production was successfully demonstrated in a process where single cells were first expanded, then cell aggregates were formed by dilution into a ...
Brian Ladd, Torbjörn Gräslund, Véronique Chotteau   +2 more
wiley   +1 more source

Series solutions for the Laguerre and Lane-Emden fractional differential equations in the sense of conformable fractional derivative

Alexandria Engineering Journal, 2019
In the present paper, we use efficient and simple algorithms of the fractional power series and Adomain polynomial methods that provide effective tools for solving such linear and nonlinear fractional differential equations in the sense of conformable ...
Zeyad Al-Zhour, Nouf Al-Mutairi, Fatimah Alrawajeh, Raed Alkhasawneh   +3 more
doaj  

A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines

Biotechnology Progress, EarlyView.
Abstract Recombinant adeno‐associated virus (rAAV) is one of the most widely used viral vectors for gene therapy. It is used in very high doses for the treatment of many diseases, making large‐scale production for clinical applications challenging. We have established a synthetic biology‐based platform to construct stable production cell lines, which ...
Yu‐Chieh Lin, Han‐Jung Kuo, Min Lu, Thomas Mahl, George Aslanidi, Wei‐Shou Hu   +5 more
wiley   +1 more source

New exact solitary wave solutions to the space-time fractional differential equations with conformable derivative

, 2019
M. Hafiz Uddin, M. Ali Akbar, Md. Ashrafuzzaman Khan, Md. Abdul Haque   +3 more
openalex   +1 more source

Continuous purification of a parvovirus using two aqueous two‐phase extraction steps

Biotechnology Progress, EarlyView.
Abstract Aqueous two‐phase systems (ATPS) are a liquid–liquid extraction method that offers low‐cost, continuous‐adaptable virus purification. A two‐step ATPS using polyethylene glycol (PEG) and sodium citrate that recovered 66% of infectious porcine parvovirus with 2.0 logs of protein removal and 1.0 logs of DNA removal in batch has now been run ...
Natalie M. Nold, Sheridan Waldack, Grace James, Trisha Colling, Lynn Manchester, Taravat Sarvari, Amanda Bekkala, Seth A. Kriz, Madison Baldwin, Emily Agustin‐Mazariegos, Michael J. Betenbaugh, Caryn L. Heldt   +11 more
wiley   +1 more source

SAAP‐148 Oligomerizes into a Hexamer Forming a Hydrophobic Inner Core

ChemBioChem, EarlyView.
The highly cationic and hydrophobic antimicrobial peptide SAP‐148 effectively eradicates multidrug‐resistant bacteria, persistent cells, and biofilms without inducing resistance. Its efficiency is likely due to its ability to disrupt bacterial membranes as an oligomer, forming a stable alpha‐helical hexameric bundle with three parallel dimers that ...
Aden Hodzic, Djenana Vejzovic, Altea Topciu, Kirill Kuhlmann, Raj Kumar, Maria Andrea Mroginski, Alejandra de Miguel, Pia Hofmann, Klaus Zangger, Markus Weingarth, Robert A. Cordfunke, Jan W. Drijfhout, Peter Nibbering, Michal Belicka, Karl Lohner, Nermina Malanovic   +15 more
wiley   +1 more source

Highly dispersive gap solitons for conformable fractional model in optical fibers with dispersive reflectivity solutions using the modified extended direct algebraic method

AIMS Mathematics
We investigated the dynamics of highly dispersive nonlinear gap solitons in optical fibers with dispersive reflectivity, utilizing a conformable fractional derivative model.
Mahmoud Soliman, Hamdy M. Ahmed, Niveen Badra, Taher A. Nofal, Islam Samir   +4 more
doaj   +1 more source

Engineering Substrate Acceptance of Resveratrol O‐Methyltransferase from Vitis vinifera for the Selective Synthesis of O‐Methyl Protected Biobased Hydroxystyrenes

ChemCatChem, EarlyView.
Reaction sequence for the synthesis of methylated products through enzymatic decarboxylation of bio‐based hydroxycinnamic acids and subsequent direct methylation of the resulting styrene intermediates without any further isolation and purification steps.
Kamela Myrtollari, Andrea M. Chánique, Daniel Kracher, Daniela P. Herrera, Joaquin Gutierrez‐Benavente, Andreas Schüller, Robert Kourist   +6 more
wiley   +1 more source

Abundant closed form solutions of the conformable time fractional Sawada-Kotera-Ito equation using (G′/G)-expansion method

Results in Physics, 2018
In current study, we use the (G′/G)-expansion method to construct the closed form solutions of the seventh order time fractional Sawada-Kotera-Ito (TFSKI) equation based on conformable fractional derivative.
Altaf Abdulkarem Al-Shawba, K.A. Gepreel, F.A. Abdullah, A. Azmi   +3 more
doaj