Results 61 to 70 of about 91,670 (264)

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Conjugal Transfer of Genetic Material in Lactococci: A Review

Journal of Dairy Science, 1989
Abstract The ability to mediate conjugal transfer of DNA is widespread among the lactococci. In the 9 yr since conjugation was first demonstrated in lactococci, a wide variety of phenotypes have been shown to be conjugally transferred. Recently, conjugation was used in constructing lactococcal strains with increased industrial utility.
James L. Steele, Larry L. McKay
openaire   +1 more source

Genetic organization of plasmid R1162 DNA involved in conjugative mobilization [PDF]

Journal of Bacteriology, 1986
DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active products and a cis-active site ...
M A, Brasch, R J, Meyer
openaire   +2 more sources

Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary

Veterinární Medicína, 2005
The presence of the chloramphenicol resistance genes cat, cmlA, flo, and the role of plasmids and class 1 integrons in the spread and persistence of chloramphenicol resistance were investigated on a collection of 40 Salmonella enterica serovar ...
N. Nogrady, I. Gado, P. Zsolt Fekete, J. Paszti   +3 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Artificial Conjugation in Escherichia coli and Transfer of Penicillinase Plasmid

پزشکی بالینی ابن سینا, 1999
Conjugation is a major mechanism of DNA transfer among bacteria which takes place in both laboratory and natural environment and brings about new characteristics. Extrachromosomal DNA called plasmid are important elements in this exchange.
Mohammad Javad Kajbaf, Mohammad Ali Mashhadizadeh, Heidar Reza Mohtadi   +2 more
doaj  

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Genetic Interactions in Streptomyces rimosus Mediated by Conjugation and by Protoplast Fusion [PDF]

Microbiology, 1983
The development of a protoplast fusion technique for oxytetracycline-producing Streptomyces rimosus strains, and its evaluation for the application for a breeding programme, has been described. Treatment of S. rimosus protoplasts with 40% (w/v) PEG 1550 for 30 min gave optimal numbers of recombinants ranging from 1 to 10% of the total progeny ...
D, Hranueli, J, Pigac, T, Smokvina, M, Alacević   +3 more
openaire   +2 more sources

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source