Results 51 to 60 of about 91,670 (264)

Genetically Directed Production of Recombinant, Isosteric and Nonhydrolysable Ubiquitin Conjugates [PDF]

ChemBioChem, 2016
AbstractWe describe the genetically directed incorporation of aminooxy functionality into recombinant proteins by using a mutant Methanosarcina barkeri pyrrolysyl‐tRNA synthetase/tRNACUA pair. This allows the general production of nonhydrolysable ubiquitin conjugates of recombinant origin by bioorthogonal oxime ligation.
Stanley, Mathew, Virdee, Satpal
openaire   +3 more sources

Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae

Microbiology Spectrum, 2022
Klebsiella pneumoniae poses a critical challenge to clinical and public health. Along with conjugative plasmids, nonconjugative resistance or virulence plasmids associated with carbapenem-resistant K. pneumoniae (CRKP), hypervirulent K. pneumoniae (hvKP),
Xiaoli Wang, Bin Tang, Guitian Liu, Meng Wang, Jingyong Sun, Ruoming Tan, Tingting Pan, Jieming Qu, Jialin Liu, Hong-Yu Ou, Hongping Qu   +10 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas [PDF]

Microbial Cell Factories, 2015
Pseudoalteromonas is commonly found throughout the world's oceans, and has gained increased attention due to the ecological and biological significance. Although over fifty Pseudoalteromonas genomes have been sequenced with an aim to explore the adaptive strategies in different habitats, in vivo studies are hampered by the lack of effective genetic ...
Wang, Pengxia, Yu, Zichao, Li, Baiyuan, Cai, Xingsheng, Zeng, Zhenshun, Chen, Xiulan, Wang, Xiaoxue   +6 more
openaire   +2 more sources

Genetically detoxified tetanus toxin as a vaccine and conjugate carrier protein

Vaccine, 2022
Tetanus toxoid (TTxd), developed over 100 years ago, is a clinically effective, legacy vaccine against tetanus. Due to the extreme potency of native tetanus toxin, manufacturing and regulatory efforts often focus on TTxd production, standardization, and safety, rather than product modernization.
Chang, Min-Ju, Ollivault-Shiflett, Morgane, Schuman, Richard, Nguyen, Son Ngoc, Kaltashov, Igor A., Bobst, Cedric, Rajagopal, Shalini P., Przedpelski, Amanda, Barbieri, Joseph T., Lees, Andrew   +9 more
openaire   +2 more sources

Genetic Dissection of a Prevalent Plasmid-Encoded Conjugation System in Lactococcus lactis

Frontiers in Microbiology, 2021
Plasmid pNP40, which was first identified nearly 40 years ago in Lactococcus lactis subsp. lactis biovar diacetylactis DRC3, encodes functions such as heavy metal-, bacteriophage-, and nisin-resistance, as well as plasmid transfer ability by conjugation.
Guillermo Ortiz Charneco, Philip Kelleher, Andrius Buivydas, Hugo Streekstra, Emiel Ver Loren van Themaat, Paul P. de Waal, Jennifer Mahony, Douwe van Sinderen   +7 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Methods for the genetic manipulation of marine bacteria

Electronic Journal of Biotechnology, 2018
Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the
Zahraa Zeaiter, Francesca Mapelli, Elena Crotti, Sara Borin   +3 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Influence of Cultivation Conditions on the Transmission of Antibiotic Resistance Genes in Vibrio cholera

Антибиотики и Химиотерапия
The growth of antibiotic resistance necessitates studying the processes of acquisition and loss of genetic elements responsible for resistance. The aim of the study was to investigate the effect of temperature, biofilm formation, and antibiotics on the ...
N. A. Selyanskaya, S. V. Titova, E. A. Menshikova, S. O. Vodopyanov, V. D. Kruglikov   +4 more
doaj   +1 more source