Results 31 to 40 of about 2,711,142 (273)

Controlling fatigue crack paths for crack surface marking and growth investigations

open access: yesFracture and Structural Integrity, 2015
While it is well known that fatigue crack growth in metals that display confined slip, such as high strength aluminium alloys, develop crack paths that are responsive to the loading direction and the local microstructural orientation, it is less well ...
S. Barter, P. White, M. Burchill
doaj   +3 more sources

Fatigue Properties of Plain Concrete under Triaxial Tension-Compression-Compression Cyclic Loading

open access: yesShock and Vibration, 2017
Fatigue tests were performed on plain concrete under triaxial tension-compression-compression (T-C-C) cyclic loading with constant and variable amplitude using a large multiaxial machine.
Dongfu Zhao   +4 more
doaj   +1 more source

Research on the vibration test load spectrum for bogie frame-mounted tractor motor based on on-track tests

open access: yes机车电传动, 2023
Ignoring the effects of high-frequency signals and small-amplitude cycles that contribute little to structural fatigue damage, rainflow counting was performed on the vibration load of traction motors to generate a three-dimensional load spectrum.
LI Guoshun   +4 more
doaj  

Cyclic Deformation and Low-Cycle Fatigue for 316LN Stainless Steel under Non-proportional Loading [PDF]

open access: yesMATEC Web of Conferences, 2019
The effects of loading path and strain amplitude ratio on the cyclic behavior and fatigue life were investigated on a 316LN nuclear grade stainless steel employing a series of symmetrically strain-controlled fatigue tests at room temperature. The loading
Li Yajing, Ren Bin, Chen Xu
doaj   +1 more source

Fatigue Life Analysis of Automotive Cast Iron Knuckle under Constant and Variable Amplitude Loading Conditions

open access: yesApplied Mechanics, 2022
The main aim of the present paper is to assess the fatigue lifetime of ductile cast iron knuckles as one of the critical components of an automotive steering system.
Kazem Reza Kashyzadeh   +4 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

A new fatigue equation for asphalt mixtures considering loading sequence effects

open access: yesCase Studies in Construction Materials
The fatigue life of asphalt mixture has been found to significantly depend on loading sequences. However, the widely used traditional linear fatigue equations are difficult to determine the fatigue life of asphalt mixture subjected to different loading ...
Chenze Fang   +5 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

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