Results 71 to 80 of about 54,888 (266)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

The Last Days of Imperial Power by A. Blok: from a Service Assignment to an Essay [PDF]

open access: yesStudia Litterarum
This article examines a number of issues related to the history of the creation of A. Blok’s essay The Last Days of Imperial Power, which was the result of the poet’s service in the Extraordinary Investigative Commission (EIC).
Alexandr S. Alexandrov
doaj   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

open access: yesFEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima   +7 more
wiley   +1 more source

Costruire la sovranità popolare. Le commissioni municipali romane e le elezioni per la Costituente del 1849 [PDF]

open access: yesLaboratorio dell'ISPF, 2012
[Constructing Popular Sovereignty. Roman Municipal Commissions and the Elections for the Constituent Assembly of 1849]. This article resumes one of the major moments of popular involvement in the Italian Risorgimento, that is, the elections for the ...
ARMANDO, David
doaj  

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Da redemocratização do Brasil através das Constituições de 1946 e 1988

open access: yesAraucaria, 2011
This paper analyzes the process of re-democratization in Brazil through the Constitution of 1946 and the Constitution of 1988.Both constitutions represented a turning point from dictatorship to democracy to establish a democratic state.
Simon Riemann Costa e Silva
doaj  

Local self-government in the Kingdom of Serbs, Croats and Slovenes [PDF]

open access: yesZbornik Radova: Pravni Fakultet u Novom Sadu, 2017
Different views on the form of state organization emerged during the session of the Constituent Assembly in the newly formed Yugoslav state. This caused the formation of two political blocs in the Constituent Assembly, the centralist and the federalist ...
Drakić Gordana M., Stanković Uroš N.
doaj  

The Atg1–kinase complex tethers Atg9-vesicles to initiate autophagy

open access: yesNature Communications, 2016
Autophagosome biogenesis is initiated by recruitment of Atg9-vesicles to the phagophore assembly site. Here Rao et al. use a reconstituted in vitrosystem to describe the mechanism by which activation of the Atg1-kinase complex from its constituent parts ...
Yijian Rao   +4 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

open access: yesAging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

The Importance of the Sources of Law Used in Drafting the Constitution of the Republic of Latvia with the Purpose of Strengthening Constitutionalism

open access: yesGdańskie Studia Prawnicze
The Constitution of the Republic of Latvia took two years to draft and entered into force on 7 November 1922. The process was influenced by historical events and the political situation in which the Latvian people found themselves, thus necessitating the
Linda Lielbriede
doaj   +1 more source

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