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Cytological differentiation of constitutive heterochromatin
Chromosoma, 1974The constitutive heterochromatin, as demonstrated by the C band technique, may be subdivided into a number of categories when other characteristics are considered. The responses to fluorochromes QM and 33258 Hoechst, the behavior following G band staining, the repetitive DNA content, and many other criteria are useful for the classification of ...
S M, Jalal +3 more
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Lateral asymmetry in human constitutive heterochromatin
Chromosoma, 1975Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromosome 9 both sister chromatids stained
R R, Angell, P A, Jacobs
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The constitutive heterochromatin of porcine chromosomes
Journal of Heredity, 1982The constitutive heterochromatin of porcine chromosomes was cytologically resolved into four types by using a combination of both C-banding techniques and base specific DNA binding fluorochromes. The four types of constitutive heterochromatin are: 1) C-band positive, A-T rich heterochromatin located in the centromeric region of acrocentric chromosomes;
C C, Lin +3 more
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Characterization of human chromosomal constitutive heterochromatin
Canadian Journal of Genetics and Cytology, 1986The constitutive heterochromatin of human chromosomes is evaluated by various selective staining techniques, i.e., CBG, G-11, distamycin A plus 4,6-diamidino-2-phenylindole-2-HCl (DA/DAPI), the fluorochrome D287/170, and Giemsa staining following the treatments with restriction endonucleases AluI and HaeIII.
A, Babu, R S, Verma
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Distribution of constitutive heterochromatin in carnivores
Cytogenetic and Genome Research, 1977The distribution of constitutive heterochromatin (C-banding) in the genomes of nine species of Felidae, one species of Mustelidae, two species of Procyonidae, one species of Viverridae, one species of Canidae, and one species of Ursidae has been studied. In the chromosomes of several felid species the C-band is apparently absent. A few chromosomes show
S, Pathak, D H, Wurster-Hill
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5-Methylcytosine localised in mammalian constitutive heterochromatin
Nature, 1974DEOXY-5-METHYLCYTIDYLIC acid (5-MeC) is an almost universal, although minor component of DNA in plants and animals. It makes up 4–7% of the bases in plants1, no more than 1.5% of the bases in the mouse2 and even less in the human3. 5-MeC itself is not incorporated directly into DNA.
Miller, O J +3 more
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Estimating percentage constitutive heterochromatin by flow cytometry
Experimental Cell Research, 1992Flow cytometry is a powerful method for the assessment of both plant and animal genomes. One of the most interesting aspects is the analysis of chromatin structure. By using intercalating and base pair-specific fluorochromes, the chromatin structure in various cell cultures and microorganisms has been determined.
A L, Rayburn, J A, Auger, L M, McMurphy
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Distribution of constitutive heterochromatin in mammalian chromosomes
Chromosoma, 1971Using a special staining technique, a survey of the chromosomes of many mammalian species showed that constitutive heterochromatin is present in all cases and that the heterochromatin pattern appears to be specific and consistent or each chromosome and each taxon.
T C, Hsu, F E, Arrighi
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Constitutive heterochromatin polymorphisms in patients with malignant diseases
Cancer, 1981The frequency of constitutive heterochromatin heteromorphisms was assessed in peripheral blood cultures of 23 patients with various malignant diseases and in their respective controls, formed by cancer-free same sex siblings of the propositi. The results showed no significant differences between the cancer and the control groups, except for chromosome ...
L, Aguilar +3 more
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CONSTITUTIVE HETEROCHROMATIN IN MOUSE CHROMOSOMES TREATED WITH TRYPSIN
Canadian Journal of Genetics and Cytology, 1973Chromosome preparations from three sublines of mouse L-cells (A9, B82 L60) were made by conventional air-drying methods and the slides treated both by trypsin digestion and the C-banding methods, in order to investigate and compare the distribution of constitutive heterochromatin in these cells.
M, Ray, J L, Hamerton
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