Results 191 to 200 of about 12,566 (212)
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Estimating percentage constitutive heterochromatin by flow cytometry
Experimental Cell Research, 1992Flow cytometry is a powerful method for the assessment of both plant and animal genomes. One of the most interesting aspects is the analysis of chromatin structure. By using intercalating and base pair-specific fluorochromes, the chromatin structure in various cell cultures and microorganisms has been determined.
A L, Rayburn, J A, Auger, L M, McMurphy
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Cytological variations in the constitutive heterochromatin of Microtus agrestis
Chromosoma, 1971In the field vole, Microtus agrestis, most of the constitutive heterochromatin is localized in the giant chromosomes. A detailed examination of a large number of adult cell types reveals that this chromatin is actually present as a heterochromatic fiber in all interphase nuclei. Depending upon the cell types, however, the fiber shows varying degrees of
J C, Lee, J J, Yunis
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Localization of mouse satellite DNA in constitutive heterochromatin
Experimental Cell Research, 1970Abstract Sonication of nuclei from the liver and brain of male mice yields three chromatin fractions with different sedimentation characteristics: a constitutive heterochromatin fraction which sediments at 3500 g, an intermediate chromatin fraction which sediments at 78,000 g and a euchromatin fraction which is recovered by precipitation with ethanol.
W G, Yasmineh, J J, Yunis
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Transgenerational epigenetic control of constitutive heterochromatin, transposons, and centromeres
Current Opinion in Genetics & Development, 2023Epigenetic mechanisms are important not only for development but also for genome stability and chromosome dynamics. The latter types of epigenetic controls can often be transgenerational. Here, we review recent progress in two examples of transgenerational epigenetic control: i) the control of constitutive heterochromatin and transposable elements and ...
Tatsuo Fukagawa, Tetsuji Kakutani
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Lateral asymmetry of constitutive heterochromatin in human chromosomes
Human Genetics, 1979Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 x SSC, and ...
P K, Ghosh, R, Rani, R, Nand
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Constitutive heterochromatin: a surprising variety of expressed sequences
Chromosoma, 2009The organization of chromosomes into euchromatin and heterochromatin is amongst the most important and enigmatic aspects of genome evolution. Constitutive heterochromatin is a basic yet still poorly understood component of eukaryotic chromosomes, and its molecular characterization by means of standard genomic approaches is intrinsically difficult ...
Dimitri P +5 more
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Cytogenetic and Genome Research, 1994
The <i>Drosophila</i> HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts ...
K A, Wreggett +5 more
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The <i>Drosophila</i> HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts ...
K A, Wreggett +5 more
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Human Genetics, 1979
Using DAPI staining after pretreatment with distamycin A we detected a familial deficiency of chromosome 16 heterochromatin. A distinct positively staining band, however, was seen after C-banding. Thus, by using these different heterochromatin staining methods, heterogeneity of the constitutive heterochromatin in the centromeric region of human ...
Buys, C.H.C.M. +4 more
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Using DAPI staining after pretreatment with distamycin A we detected a familial deficiency of chromosome 16 heterochromatin. A distinct positively staining band, however, was seen after C-banding. Thus, by using these different heterochromatin staining methods, heterogeneity of the constitutive heterochromatin in the centromeric region of human ...
Buys, C.H.C.M. +4 more
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Simultaneous Staining of the Spindle Apparatus and Constitutive Heterochromatin
Stain Technology, 1974In nonhypotonically treated mitoses from tissue cultures grown on cover glasses, the constitutive heterochromatin as well as the spindle apparatus can be stained by a Giemsa technique for demonstrating repetitive DNA. Cells were treated with 0.015 M sodium chloride and 0.0015 M sodium citrate at 92 C for about 3 min, then with 03 M sodium chloride and ...
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Asynchronous replication of constitutive heterochromatin on X chromosomes in female Mus dunni
Chromosoma, 1982Euchromatin DNA of one X chromosome in mammalian females, which becomes facultatively heterochromatinized, is known to replicate asynchronously late in S phase compared to its active homologue. In the females of a pygmy mouse species Mus dunni, which has prominent segment of constitutive heterochromatin as the short arm of its submetacentric X ...
S, Sen, T, Sharma
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