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Distribution of constitutive heterochromatin in mammalian chromosomes
Chromosoma, 1971Using a special staining technique, a survey of the chromosomes of many mammalian species showed that constitutive heterochromatin is present in all cases and that the heterochromatin pattern appears to be specific and consistent or each chromosome and each taxon.
T. C. Hsu, Frances E. Arrighi
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5-Methylcytosine localised in mammalian constitutive heterochromatin
Nature, 1974DEOXY-5-METHYLCYTIDYLIC acid (5-MeC) is an almost universal, although minor component of DNA in plants and animals. It makes up 4–7% of the bases in plants1, no more than 1.5% of the bases in the mouse2 and even less in the human3. 5-MeC itself is not incorporated directly into DNA.
Miller, O J +3 more
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Constitutive heterochromatin polymorphisms in patients with malignant diseases
Cancer, 1981The frequency of constitutive heterochromatin heteromorphisms was assessed in peripheral blood cultures of 23 patients with various malignant diseases and in their respective controls, formed by cancer-free same sex siblings of the propositi. The results showed no significant differences between the cancer and the control groups, except for chromosome ...
Lydia Aguilar +3 more
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Asynchronous replication of constitutive heterochromatin on X chromosomes in female Mus dunni
Chromosoma, 1982Euchromatin DNA of one X chromosome in mammalian females, which becomes facultatively heterochromatinized, is known to replicate asynchronously late in S phase compared to its active homologue. In the females of a pygmy mouse species Mus dunni, which has prominent segment of constitutive heterochromatin as the short arm of its submetacentric X ...
S. Sen, T. Sharma
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CONSTITUTIVE HETEROCHROMATIN IN MOUSE CHROMOSOMES TREATED WITH TRYPSIN
Canadian Journal of Genetics and Cytology, 1973Chromosome preparations from three sublines of mouse L-cells (A9, B82 L60) were made by conventional air-drying methods and the slides treated both by trypsin digestion and the C-banding methods, in order to investigate and compare the distribution of constitutive heterochromatin in these cells.
J. L. Hamerton, M. Ray
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Constitutive heterochromatin in early embryogenesis of Drosophila melanogaster
Molecular and General Genetics MGG, 1991The formation of constitutive heterochromatin was studied during the embryonic development of Drosophila melanogaster, using the C-banding technique. During embryonic cleavage, C-banded material is not seen in mitotic chromosomes; the differentiation between euchromatin and heterochromatin only occurs at blastoderm.
I. E. Vlassova +3 more
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DNase I sensitivity in facultative and constitutive heterochromatin
Chromosoma, 1985In situ nick translation allows the detection of DNase I sensitive and insensitive regions in fixed mammalian mitotic chromosomes. We have determined the difference in DNase I sensitivity between the active and inactive X chromosomes in Microtus agrestis (rodent) cells, along both their euchromatic and constitutive heterochromatic regions. In addition,
Batsheva Kerem +5 more
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A developmental study of constitutive heterochromatin in Microtus agrestis
Chromosoma, 1971In the vole, Microtus agrestis, the constitutive heterochromatin is largely restricted to the giant sex chromosomes but varies in its degree of condensation in various cell types. In the cleavage embryos and fibroblasts it formed one or two long and extended heterochromatic fibers, in hepatocytes it formed two large and diffuse masses and in neurons ...
Jorge J. Yunis, J. C. Lee
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Localization of mouse satellite DNA in constitutive heterochromatin
Experimental Cell Research, 1970Abstract Sonication of nuclei from the liver and brain of male mice yields three chromatin fractions with different sedimentation characteristics: a constitutive heterochromatin fraction which sediments at 3500 g, an intermediate chromatin fraction which sediments at 78,000 g and a euchromatin fraction which is recovered by precipitation with ethanol.
W.G. Yasmineh, Jorge J. Yunis
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Estimating percentage constitutive heterochromatin by flow cytometry
Experimental Cell Research, 1992Flow cytometry is a powerful method for the assessment of both plant and animal genomes. One of the most interesting aspects is the analysis of chromatin structure. By using intercalating and base pair-specific fluorochromes, the chromatin structure in various cell cultures and microorganisms has been determined.
Julie A. Auger +2 more
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