Results 251 to 260 of about 346,273 (274)

Localization of mouse satellite DNA in constitutive heterochromatin

Experimental Cell Research, 1970
Abstract Sonication of nuclei from the liver and brain of male mice yields three chromatin fractions with different sedimentation characteristics: a constitutive heterochromatin fraction which sediments at 3500 g, an intermediate chromatin fraction which sediments at 78,000 g and a euchromatin fraction which is recovered by precipitation with ethanol.
W.G. Yasmineh, Jorge J. Yunis
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Estimating percentage constitutive heterochromatin by flow cytometry

Experimental Cell Research, 1992
Flow cytometry is a powerful method for the assessment of both plant and animal genomes. One of the most interesting aspects is the analysis of chromatin structure. By using intercalating and base pair-specific fluorochromes, the chromatin structure in various cell cultures and microorganisms has been determined.
Julie A. Auger, L. M. McMurphy, A. Lane Rayburn   +2 more
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Cytological variations in the constitutive heterochromatin of Microtus agrestis

Chromosoma, 1971
In the field vole, Microtus agrestis, most of the constitutive heterochromatin is localized in the giant chromosomes. A detailed examination of a large number of adult cell types reveals that this chromatin is actually present as a heterochromatic fiber in all interphase nuclei. Depending upon the cell types, however, the fiber shows varying degrees of
Jorge J. Yunis, J. C. Lee
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Lateral asymmetry of constitutive heterochromatin in human chromosomes

Human Genetics, 1979
Variations in lateral asymmetry of constitutive heterochromatin were studied in 30 normal individuals with reference to the chromosomal regions 1q12, 9q12, 15p11, 16q12 and Yq12. The technique consisted of growing human lymphocytes for one cell cycle in BrdU, staining with 33258 Hoechst, exposing them to UV light, treating them with 2 x SSC, and ...
Rajni Rani, Reita Nand, P.K. Ghosh
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Induced pluripotent stem-induced cells show better constitutive heterochromatin remodeling and developmental potential after nuclear transfer than their parental cells.

Stem Cells and Development, 2012
Recently, reprogramming of somatic cells from a differentiated to pluripotent state by overexpression of specific external transcription factors has been accomplished. It has been widely speculated that an undifferentiated state may make donor cells more
Zichuan Liu, Haifeng Wan, E. Wang, Xiao-Yang Zhao, C. Ding, S. Zhou, Tianda Li, L. Shuai, Chunjing Feng, Yang Yu, Qi Zhou, N. Beaujean   +11 more
semanticscholar   +1 more source

A comparison of constitutive heterochromatin staining methods in two cases of familial heterochromatin deficiencies

Human Genetics, 1979
Using DAPI staining after pretreatment with distamycin A we detected a familial deficiency of chromosome 16 heterochromatin. A distinct positively staining band, however, was seen after C-banding. Thus, by using these different heterochromatin staining methods, heterogeneity of the constitutive heterochromatin in the centromeric region of human ...
W. L. Gouw, J. M. M. Borkent-Ypma, J. A. M. Blenkers-Platter, G. J. P. A. Anders, Charles H.C.M. Buys   +4 more
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A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin

Cytogenetic and Genome Research, 1994
The <i>Drosophila</i> HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts ...
G W Butcher, Prim B. Singh, P S James, A Hutchings, F Hill, K A Wreggett   +5 more
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Simultaneous Staining of the Spindle Apparatus and Constitutive Heterochromatin

Stain Technology, 1974
In nonhypotonically treated mitoses from tissue cultures grown on cover glasses, the constitutive heterochromatin as well as the spindle apparatus can be stained by a Giemsa technique for demonstrating repetitive DNA. Cells were treated with 0.015 M sodium chloride and 0.0015 M sodium citrate at 92 C for about 3 min, then with 03 M sodium chloride and ...
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Heteromorphism of constitutive heterochromatin in carcinoma and dysplasia of the uterine cervix

European Journal of Obstetrics & Gynecology and Reproductive Biology, 1980
Chromosomal heteromorphism in constitutive heterochromatin of chromosomes 1, 9 and 16 was studied in 8 patients with cervix cancer and 15 patients with cervix dysplasia. Tissue cultures (diploid fibroblasts) established from tumor biopsies as well as lymphocyte cultures were used for making C-banded chromosome preparations.
W. K. Heneen, Z. A. Habib, D. Röhme
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Constitutive Heterochromatin Polymorphisms in Children with Acute Lymphoblastic Leukemia

Pediatric Hematology and Oncology, 1993
The C-band heterochromatin polymorphisms of chromosomes 1, 9, and 16 were studied on peripheral lymphocytes of 67 children with acute lymphoblastic leukemia (ALL) and 50 control individuals. A statistically significant difference between patients and controls was found for large heterochromatin regions (level 3) of chromosomes 1 and 9 (P < 0.001) and ...
TSEZOU, A, KITSIOUTZELI, S, KOSMIDIS, H, PAIDOUSI, K, KATSOUYANNI, K, SINANIOTIS, C   +5 more
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