Results 91 to 100 of about 35,093 (211)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Desvios de custos e prazos em empreendimentos da construção civil: categorização e fatores de influência

Ambiente Construído
Desvios de custos e prazos são desafios recorrentes na construção civil. Neste artigo, os autores objetivaram revisar sistematicamente a literatura relacionada a desvios de prazos e custos para determinar uma estrutura de categorização e respectivos ...
Elisa Atália Daniel Muianga, Ariovaldo Denis Granja, Joyce de Andrade Ruiz   +2 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Forecasting Total and Type-Specific Non-Residential Building Construction Spending: The Case Study of the United States and Lessons Learned

Buildings
Forecasting construction spending is important for civil engineering practitioners to make business decisions. Currently, the main body of forecasting literature pertains exclusively to aggregate construction investment, such as total construction ...
Xingrui Zhang, Yunpeng Wang, Shuai Xu, Eunhwa Yang, Lingxiao Meng   +4 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Broad-area-search of new construction using time series analysis of Landsat and Sentinel-2 data

Science of Remote Sensing
New construction activities can alter surface albedo and structure, which then affect surface temperature and roughness, and hence have a significant impact on urban climate.
Xiaojing Tang, Madison G. Barrett, Kangjoon Cho, Kelsee H. Bratley, Katelyn Tarrio, Yingtong Zhang, Hanfeng Gu, Peter Rasmussen, Marc Bosch, Curtis E. Woodcock   +9 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Design and Construction of Inverse Time & Definite Time Overcurrent Relay Based on Arduino Uno

Emitor: Jurnal Teknik Elektro
This system is designed to detect overcurrent faults, then disconnect them from the circuit. In inverse time overcurrent relay, the relay trip time will depend on the magnitude of the fault current.
rizky susilo, Lucianus Handri Gunanto, Ipniansyah Ipniansyah, Abdurrahim Abdurrahim, Toto Nugroho, Gusti Fikri Fadhillah, Muhammad Ary Sandy Saputra   +6 more
doaj   +1 more source