Results 51 to 60 of about 461,033 (264)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

open access: yesMolecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen   +11 more
wiley   +1 more source

Content and face validity in virtual reality with children: a validation in five steps+1 of a wheelchair basketball game

open access: yesFrontiers in Virtual Reality
ObjectiveThe present study is aimed to elaborate and determinate the content and face validity of a virtual reality program attending the perspective of children.
Imanol Ceberio   +7 more
doaj   +1 more source

Development and psychometric analysis of a new tool to assess food literacy in diabetic patients

open access: yesBMC Nutrition, 2022
Background One of the factors affecting self-care in diabetic patients is food literacy, which helps said patients in following a healthy diet. Thus, it is crucial to analyze food literacy in diabetic patients through suitable and reliable instruments ...
Fatemeh Bastami   +2 more
doaj   +1 more source

Dual PI3K/AKT and CDK4/6 inhibition reveals selective sensitivity in an SHH medulloblastoma stem cell model

open access: yesMolecular Oncology, EarlyView.
Targeted therapy was evaluated in SHH medulloblastoma using neuroepithelial stem cell (NES) and tumor‐derived NES‐like (tNES) models in 2D monolayers and 3D spheroids. PI3K, AKT, and CDK4/6 inhibitors had minimal effects in NES but markedly reduced viability and growth and induced apoptosis in tNES cells, revealing distinct therapeutic vulnerabilities.
Monika Lukoseviciute   +4 more
wiley   +1 more source

Development and Validation of a Higher-Order Thinking Skills-Based Test on Citizen Participation in Consumer Education Using Item Response Theory

open access: yesJISAE (Journal of Indonesian Student Assessment and Evaluation)
This study aims to develop and validate a high-order thinking skills (HOTS)-based multiple-choice instrument to assess citizen participation in consumer education.
Putu Diah Ari Kusumadewi   +3 more
doaj   +1 more source

Development of a New Patient-Reported Medication Adherence Instrument: Concerns Influencing Medication Adherence

open access: yesPatient Preference and Adherence, 2021
Sonal Ghura Mansukhani,1 Elizabeth A MacLean,2 Laura L Manzey,2 Carl J Possidente,2 Joseph C Cappelleri,2 Linda S Deal2 1Patient Centered Outcomes Research, Evidera, Waltham, Massachusetts, USA; 2Medical Outcomes Specialists (EAM, LLM, CJP); Patient ...
Mansukhani SG   +5 more
doaj  

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

open access: yesFEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini   +2 more
wiley   +1 more source

Content Validity and Reliability of the Measurement Tools in Educational, Behavioral, and Health Sciences Research

open access: yesJournal of Medical Education Development, 2018
Development and assessment of measurement tools are important stages in the research processes regarding social, educational, and medical sciences, which mainly focus on the measurement of characteristics, qualitative variables, and abstract variables ...
Mohammad Masoud Vakili, Nasrin Jahangiri
doaj  

Overview of molecular signatures of senescence and associated resources: pros and cons

open access: yesFEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas   +6 more
wiley   +1 more source

Antibiofilm activity of a chionodracine‐derived peptide by NMR‐based metabolomics of cell‐free supernatant of Acinetobacter baumannii clinical strains

open access: yesFEBS Open Bio, EarlyView.
KHS‐Cnd peptide is able to impair biofilm formation and disaggregate mature biofilms in Acinetobacter baumannii clinical isolates. Differences in extracellular metabolites reflect changes in biofilm metabolism due to KHS‐Cnd treatment. Among the differentially represented extracellular metabolites upon KHS‐Cnd treatment, the significantly altered ...
Fernando Porcelli   +9 more
wiley   +1 more source

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