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Specificity of a coproantigen ELISA test for fasciolosis: lack of cross‐reactivity with Paramphistomum cervi and Taenia hydatigena

Veterinary Record, 2012
A commercial coproantigen ELISA test for fasciolosis, based on the use of MM3 monoclonal antibody for antigen capture, was investigated for possible cross‐reactivity with Paramphistomum cervi , a trematode that commonly infects cattle and sheep ...
P-E, Kajugu   +6 more
openaire   +2 more sources

AN ULTRASENSITIVE CAPTURE ELISA FOR DETECTION OF FASCIOLA HEPATICA COPROANTIGENS IN SHEEP AND CATTLE USING A NEW MONOCLONAL ANTIBODY (MM3)

Journal of Parasitology, 2004
A capture enzyme-linked immunosorbent assay (ELISA) using a new monoclonal antibody (mAb MM3) is reported for the detection of Fasciola hepatica excretory-secretory antigens (ESAs) in feces of infected hosts. The mAb MM3 was produced by immunization of mice with a 7- to 40-kDa purified and O-deglycosylated fraction of F.
Mercedes, Mezo   +3 more
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Comparison of early detection of Fasciola hepatica in experimentally infected Merino sheep by real-time PCR, coproantigen ELISA and sedimentation

Veterinary Parasitology, 2018
Fasciolosis due to infection with Fasciola hepatica, Fasciola gigantica or their hybrids is a significant global cause of livestock production loss. Infection is commonly diagnosed by a labour-intensive sedimentation and faecal egg count (FEC), which has limited throughput and is only applicable after completion of the 8-12 week pre-patent period (PPP).
Nichola Eliza Davies, Calvani   +4 more
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MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica

Veterinary Parasitology, 2009
During an experimental infection of sheep with Fasciola hepatica or F. gigantica, MM3-SERO and MM3-COPRO ELISA tests were applied to compare the kinetics of antibody production and coproantigen release between the 2nd and 32nd week post-infection (wpi).
M Adela, Valero   +8 more
openaire   +2 more sources

Detection of coproantigens by sandwich ELISA in sheep experimentally infected with Fasciola gigantica.

Tropical biomedicine, 2006
Two monoclonal antibodies to Fasciola gigantica excretory/secretory (ES) antigens were used in a sandwich ELISA for the detection of Fasciola antigens in faeces of 9 sheep experimentally infected with 300 metacercariae of F. gigantica. The detection of coproantigens was found in four of the seven sheep within 5 weeks of infection, and within 7 weeks of
S, Endah Estuningsih   +3 more
openaire   +1 more source

Assessment of the potential of three Haemonchus contortus glutathione S-transferase (HcGST) isozymes for the development of a coproantigen capture ELISA diagnostic

2023
Submission note: A thesis submitted in total fulfilment of Doctor of Philosophy to the Department of Genetics, School of Molecular Sciences, Faculty of Science, Technology and Engineering, La Trobe University, Bundoora, Victoria.The public version of this thesis does not contain all material submitted for examination.
openaire   +1 more source

Cross-Reactivity of Some Cryptosporidium Species with Cryptosporidium parvum Coproantigen in a Commercial ELISA Kit

2011
To obtain information about the prevalence of Cryptosporidium parvum in cattle in Oyo State, Nigeria, a commercially available enzyme-linked immunosorbent assay (ELISA) kit made from Cryptosporidium-specificantibodies raised against antigens of Cryptosporidium parvum was used to screen 406 randomly collected fecal samples from cattle in four White ...
Ayinmode, AB, Fagbemi, BO
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ELISA-based coproantigen in human strongyloidiaisis: a diagnostic method correlating with worm burden.

Journal of the Egyptian Society of Parasitology, 2010
In order to overcome the false negative diagnosis of strongyloidiasis in the absence of rhabditiform larvae in stools, an ELISA-based Strongyloides stercoralis-specific coproantigen detection assay in stools of infected patients was evaluated. In a sandwich ELISA, a rabbit hyperimmune serum against S.
openaire   +1 more source

Comparison of conventional coproscopical methods and commercial coproantigen ELISA kits for the detection of Giardia and Cryptosporidium infections in dogs and cats.

Berliner und Munchener tierarztliche Wochenschrift, 2005
Faecal samples of 270 dogs and 100 cats from 3 animal shelters in Germany were comparatively examined using conventional coproscopical methods and commercial coproantigen ELISA kits for the detection of Giardia and Cryptosporidium infections. Giardia cysts were found in 9.5% and 0% of the faecal samples in dogs and cats, respectively, examined once ...
Veli Y, Cirak, Christian, Bauer
openaire   +1 more source

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