Results 151 to 160 of about 12,093,925 (280)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Tobacco Industry's Corporate Diversification in the Evolving Landscape of Tobacco Control Laws in India - A Comparative Time-Series Analysis. [PDF]

Indian J Community Med
Khaitan A, Kapoor S, Yadav A, Madan C.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Sex-associated differences in lesion delivery and atrial fibrillation recurrence after cryoballoon ablation. [PDF]

Europace
Heil E, Bock M, Knocks J, Rudolph JP, Gerds-Li JH, Heinzel F, Attanasio P, Parwani AS, Boldt H, Blaschke F, Schöppenthau D, Lueg JC, Althoff T, Dagres N, Tscholl V, Hindricks G, Hohendanner F.   +16 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Benefits of hypocrisy: Do managers gain more from greenwashing. [PDF]

PLoS One
Dong W, Yang Y, Chen Y.
europepmc   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Replication and compartmentalization of Dobrava-Belgrade virus in the central nervous system. [PDF]

Emerg Microbes Infect
Melchert J, Herzig-Nichtweiss J, Meinhardt J, Salih F, Best TD, Schmidt ML, Müller LJ, Veith T, Beheim-Schwarzbach J, Hofmann J, Jones TC, Siebert E, Schoenrath F, Radbruch H, Drosten C, Corman VM.   +15 more
europepmc   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Septin multimer autoantibodies in severe motor neuropathy mimicking lower motor neuron disease. [PDF]

Brain
Arlt FA, Miske R, Appeltshauser L, Zinnow V, Borowski K, Stenzel W, Radbruch H, Meisel A, Ruprecht K, Endres M, Blau I, Kirchner M, Mertins P, Sanchez-Sendin E, Wernick S, Pressler H, Stascheit F, Linke J, Hümmert S, Werner HB, Wibisono EA, Doppler K, Komorowski L, Spiliotis ET, Dubey D, Zeckeridou A, Pittock SJ, Mills JR, McKeon A, Scharf M, Prüss H.   +30 more
europepmc   +1 more source