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Determination of endogenous corticosterone in rodent’s blood, brain and hair with LC–APCI–MS/MS

open access: yesJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2015
Endogenous corticosterone in rodent's hair would be a potential biomarker to assess the response of the hypothalamus-pituitary-adrenal axis to chronic stress.
Wei-Wen Wang, Huihua Deng, Zheng Chen
exaly   +2 more sources
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Corticosterone in Chicken Eggs

Annals of the New York Academy of Sciences, 2005
Abstract: Birds are discussed as models for prenatal stress. In this study, several experiments were conducted to gain basic knowledge of if, how, and when maternal adrenocortical activity is reflected by corticosterone concentrations in the egg. Radiolabeled corticosterone was administered to 10 laying hens to investigate the uptake into as well as ...
S, Rettenbacher   +3 more
openaire   +2 more sources

Rapid corticosterone pulses

Journal of Steroid Biochemistry, 1983
If micro-sampling techniques are used in conjunction with a highly sensitive radioimmunoassay procedure, it is possible to obtain repeated rapid measurements of plasma corticosterone concentrations from unanesthetized rats. Such a procedure reveals the existence of rapid corticosterone pulses with a duration of less than 60 s and amplitudes as great as
openaire   +2 more sources

Enzyme immunoassay of corticosterone

Journal of Steroid Biochemistry, 1979
Abstract Enzyme immunoassay of corticosterone was developed using alkaline phosphatase as a labelling enzyme. Serum corticosterone was measured by this system after the extraction and purification of corticosterone by Sephadex LH-20 column chromatography.
Y, Kobayashi   +4 more
openaire   +2 more sources

A radioimmunoassay for plasma corticosterone

Steroids, 1972
Abstract A radioimmunoassay for plasma corticosterone has been developed. Antiserum against corticosterone was produced in rabbits immunized with corticosterone -21- hemisuccinate conjugated to bovine serum albumin. The assay was practical and reliable. The coefficient of variation between assays was ± 23% and among assays was ± 8%.
H A, Gross   +3 more
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Corticosterone rhythm of mouse adrenal in relation to serum corticosterone and sampling

American Journal of Physiology-Legacy Content, 1959
Under conditions standardized for light-synchronized periodicity analysis the corticosterone content of mouse adrenals undergoes significant 24-hour periodic changes. In mature males of the B1 stock, glandular hormone content fluctuates by approximately 50% around its day-mean, accounting amplitude-wise for about one-half of the periodic change in ...
F, HALBERG, P G, ALBRECHT, J J, BITTNER
openaire   +2 more sources

Cannabinoid effects on plasma corticosterone and uptake of 3H-corticosterone by mouse brain

European Journal of Pharmacology, 1978
The effects of three cannabinoids, 11-hydroxy-delta9-tetrahydrocannabinol (11-HO-delta9-THC), delta9-THC and cannabinol (CBN), ranging in behavioral activity from high to low, were studied on two aspects of pituitary--adrenal function. Plasma corticosterone levels were used as an index of adrenocorticotropic hormone (ACTH) release.
K M, Johnson   +3 more
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The release of corticosterone and a corticosterone-binding protein by incubated rat adrenal slices

The Journal of Steroid Biochemistry and Molecular Biology, 1991
Stimulation of incubated rat adrenal slices with ACTH(1-24) resulted in an increase in the release of both corticosterone and specific corticosterone-binding protein into the incubation medium. The release of corticosterone and binding protein was dose and calcium dependent with adrenals from animals pretreated with betamethasone.
S, Ali, J R, Bassett
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Fecal corticosterone metabolites and plasma corticosterone in Japanese quail selected for low or high plasma corticosterone responses to brief restraint

Domestic Animal Endocrinology, 2012
Fecal corticosterone metabolites and plasma corticosterone in Japanese quail selected for low- or high-plasma corticosterone responses to brief mechanical restraint (low- and high-stress lines), and in a line of unselected quail, were measured in this study.
J F, Cockrem   +3 more
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BINDING OF CORTICOSTERONE IN RAT LIVER

Journal of Endocrinology, 1970
SUMMARY The binding characteristics of corticosterone by rat liver were studied by a displaceable binding technique. The binding of corticosterone to protein fractionated by gel filtration and density gradient centrifugation has been carried out as a preliminary determination of the nature of the binding sites.
R S, Snart, N N, Sanyal, M K, Agarwal
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