Results 51 to 60 of about 5,031,599 (281)
IEEE Access, 2021 Software organization always aims at developing a quality software product using the estimated development resources, effort, and time. Global Software Development (GSD) has emerged as an essential tool to ensure optimal utilization of resources, which ...
Junaid Ali Khan +3 more
doaj +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Modelling stem cell differentiation related processes—A practical overview for biologists
FEBS Letters, EarlyView.Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar +4 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Cost Data Modelling and Searching to Support Low Volume, High Complexity, Long Life Defence Systems Development [PDF]
, 2015 This paper presents a data modelling and a semi-automatic data searching method to support cost estimation in the product development process, particularly for low volume, high complexity and long life products typified by defence products and systems ...
Marsh, Robert +4 more
core +1 more source
A price-determining model for industrial “technology-push” innovations [PDF]
Innovative MarketingType of the article: Research Article AbstractThe complexity of industrial markets in the Fourth Industrial Revolution necessitates new pricing approaches for R&D outcomes.
Nataliya Chukhray +3 more
doaj +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
Molecular Oncology, EarlyView.Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur +11 more
wiley +1 more source
Preliminary project cost estimation model using artificial neural networks for public sector office buildings in Sri lanka. [PDF]
, 2015 Cost estimating is a critical due to incomplete project details and drawings and has become a similar issue in Sri Lanka. Since, cost of a building is impacted by decisions made at the design phase, efficient cost estimation is essential. Therefore novel
Fernando, Nirodha +3 more
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