Results 161 to 170 of about 1,586,292 (228)

High-Speed Countercurrent Chromatography

Critical Reviews in Analytical Chemistry, 1986
I. INTRODUCTION During the past 20 years countercurrent chromatography (CCC) has been steadily improved in both partition efficiency and separation times.1-15 The original helix CCC and the successively developed nonhelix preparative-scale CCC schemes, such as droplet CCC16,17 and locular CCC,17 required relatively long separating times; sizeable ...
Yoichiro Ito, Walter D. Conway
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Isolation of astaxanthin monoesters from the microalgae Haematococcus pluvialis by high performance countercurrent chromatography (HPCCC) combined with high performance liquid chromatography (HPLC)

, 2020
Astaxanthin is a potent natural antioxidant with beneficial bioactivities demonstrated primarily for its free (non-esterified) form. However, its natural producer, the microalgae Haematococcus pluvialis synthesizes astaxanthin mostly in ester forms which
T. Fábryová, L. Tumova, Daniela Correia da Silva, D. Pereira, P. Andrade, P. Valentão, P. Hrouzek, J. Kopecký, J. Cheel   +8 more
semanticscholar   +1 more source

Separation of six antioxidants from Hypsizygus marmoreus by high-speed countercurrent chromatography utilizing an approach based upon the polarity parameter model.

Journal of Chromatography A, 2020
For a successful high-speed countercurrent chromatography (HSCCC) separation of multiple components, the suitable solvent system selection is a critical operation.
Xu Cai, Mi Xiao, Xian-Wei Zou, Jin-Tian Tang, Bi-Sheng Huang, Hong-Kun Xue   +5 more
semanticscholar   +1 more source

Integrative countercurrent chromatography for the target isolation of lysine-specific demethylase 1 inhibitors from the roots of Salvia miltiorrhiza.

Talanta: The International Journal of Pure and Applied Analytical Chemistry, 2020
The ability to separate bioactive compounds from herbal medicines, which contain abundant components, is crucial for drug discovery. Conventional Countercurrent chromatography (CCC) methods for separating bioactive compounds are labor intensive and show ...
Yaolan Lin, Chao Han, Qi-Qi Xu, Wenli Wang, Ling-Nan Li, Dongrong Zhu, Jian-Guang Luo, Ling-Yi Kong   +7 more
semanticscholar   +1 more source

pH-zone-refining countercurrent chromatography

Journal of Chromatography A, 1996
A recently developed preparative technique, pH-zone-refining countercurrent chromatography (CCC), separates organic acids and bases according to their pKa and hydrophobicity. The hydrodynamic mechanism of pH-zone-refining CCC is illustrated in two elution modes along with a simple mathematical analysis.
Yoichiro Ito
exaly   +3 more sources

Separation of betacyanins from Iresine herbstii Hook. ex Lindl. leaves by high-speed countercurrent chromatography in a polar solvent system.

Journal of Chromatography A, 2020
Betacyanins, natural plant pigments, from Iresine herbstii Hook. ex Lindl. leaf extract were separated for the first time by high-speed countercurrent chromatography (HSCCC) in a highly polar solvent system composed of PrOH-ACN-(NH4)2SO4satd.soln-H2O (1 ...
A. Spórna-Kucab, Natalia Wróbel, Agnieszka Kumorkiewicz-Jamro, S. Wybraniec   +3 more
semanticscholar   +1 more source

A rapid and practical prediction method for the Arizona solvent system family used in high speed countercurrent chromatography.

Journal of Chromatography A, 2020
Selecting the appropriate solvent system is the key to the successful separation of samples by using countercurrent chromatography. Although high-speed countercurrent chromatography has been widely used in the separation and preparation of natural ...
Xu Wang, Chen Liu, Qingyun Ma, Zhen-Hua Tian, Hai-Qiang Jiang, Qingtao Lv, Rong Rong   +6 more
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Advances in Countercurrent Chromatography For Protein Separations

Bioanalysis, 2012
Countercurrent chromatography (CCC) is a preparative purification technique working with biphasic liquid systems. One phase of the liquid system is selected to be the mobile phase, the other phase is the stationary phase, held still by centrifugal fields. Aqueous two-phase systems (ATPS) have demonstrated their use in biological purifications.
Mekaoui, Nazim, Faure, Karine, Berthod, Alain   +2 more
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A biphasic system based on guanidinium ionic liquid: Preparative separation of eicosapentaenoic acid ethyl ester and docosahexaenoic acid ethyl ester by countercurrent chromatography.

Journal of Chromatography A, 2020
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are high nutritional components. Evidence for unique effects of them is increasing. Further understanding of their independent biological functions urgently needs more efficient separation ...
C. Fan, Lijiao Wen, Xue-Li Cao
semanticscholar   +1 more source

Dual Countercurrent Chromatography

Journal of Liquid Chromatography, 1988
Abstract A modern method of performing conventional countercurrent distribution called Dual Countercurrent Chromatography is described. The system consists of a multilayer coiled column integrated with two inlet and two outlet lines for solvent phases and a sample feed line. Subjecting the system to a particular combination of centrifugal and planetary
Y.-W. Lee, C. E. Cook, Y. Ito
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