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Recycling Foam Countercurrent Chromatography
Analytical Chemistry, 2000A new sample injection method for foam countercurrent chromatography (CCC), named the "recycle injection system", has been developed. In this system, the effluent from the liquid outlet is directly returned into the column through the sample feed line so that the sample solution is continuously recycled for repetitive foam fractionation. The utility of
, Oka +4 more
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High-Speed Countercurrent Chromatography
Critical Reviews in Analytical Chemistry, 1986I. INTRODUCTION During the past 20 years countercurrent chromatography (CCC) has been steadily improved in both partition efficiency and separation times.1-15 The original helix CCC and the successively developed nonhelix preparative-scale CCC schemes, such as droplet CCC16,17 and locular CCC,17 required relatively long separating times; sizeable ...
Yoichiro Ito, Walter D. Conway
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Dual Countercurrent Chromatography
Journal of Liquid Chromatography, 1988Abstract A modern method of performing conventional countercurrent distribution called Dual Countercurrent Chromatography is described. The system consists of a multilayer coiled column integrated with two inlet and two outlet lines for solvent phases and a sample feed line. Subjecting the system to a particular combination of centrifugal and planetary
Y.-W. Lee, C. E. Cook, Y. Ito
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Progress of chiral countercurrent chromatography
Chinese Journal of Chromatography, 2016This article summarizes five characteristics of chiral countercurrent chromatography (CCC). The progress of chiral countercurrent chromatography is introduced. In the high speed countercurrent chromatography, the chiral selectors such as amino acid derivatives, cyclodextrin derivatives, chiral organic acid, polysaccharide and bovine serum albumin (BSA)
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Advances in Countercurrent Chromatography For Protein Separations
Bioanalysis, 2012Countercurrent chromatography (CCC) is a preparative purification technique working with biphasic liquid systems. One phase of the liquid system is selected to be the mobile phase, the other phase is the stationary phase, held still by centrifugal fields. Aqueous two-phase systems (ATPS) have demonstrated their use in biological purifications.
Mekaoui, Nazim +2 more
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multifraction separation in countercurrent chromatography (MCSGP)
Biotechnology and Bioengineering, 2013ABSTRACTThe multicolumn countercurrent solvent gradient purification (MCSGP) process is a continuous countercurrent multicolumn chromatography process capable of performing three fraction separations while applying a linear gradient of some modifier.
M. Krättli +2 more
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Hydrophobic and hydrophilic interactions in countercurrent chromatography
Journal of Chromatography A, 2020Countercurrent chromatography (or counter-current chromatography, CCC) is a unique support-free liquid-liquid partition chromatography. Since it was invented by Y. Ito in 1960s, CCC has been widely accepted and applied as popular separation and purification technique for natural and synthetic complex.
Lihong Zhang, Shihua Wu
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Resolution in Countercurrent Chromatography
Journal of Liquid Chromatography, 1985Abstract Using as an example the separation of DNP-glutamic acid and DNP-alanine in a nonplanetary countercurrent chromatograph, it was demonstrated that resolution, Rs, of a solute pair can be satisfactorily predicted from knowledge of the column efficiency, N, the fraction of column volume occupied by stationary phase, SF, and the partition ...
Walter D. Conway, Yoichiro Ito
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High-speed countercurrent chromatography
Nature, 1987Support-free high-speed countercurrent liquid chromatography provides a rich domain of applications, some beyond reach of conventional liquid chromatography.
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Comparison of Countercurrent Chromatography with Flash Chromatography
Journal of Liquid Chromatography & Related Technologies, 1998Abstract Studies of the separation of benzyl, phenylethyl and phenylpropyl alcohols by flash chromatography (FC) on 1.0 × 30 or 1.9 × 15 cm silica gel columns are compared with separation by countercurrent chromatography (CCC) on a 1.68 mm × 24 m, 56 mL, PTFE multilayer coil.
W. D. Conway +3 more
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