Results 11 to 20 of about 3,518 (185)

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification. [PDF]

Biotechnol Bioeng
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Wang Z, Javidanbardan A, Behboudi A, Zydney AL.   +3 more
europepmc   +2 more sources

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP). [PDF]

Biotechnol Prog
Abstract High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical ...
Mohammadzadehmarandi A, Heller N, Ahmadimashhadi M, Petrone J, Marchand N, Darvishmanesh S, Zhang K, Menegatti S, Zydney AL.   +8 more
europepmc   +2 more sources

Impact of Air–Sea Turbulent Heat Flux on Eddy-Induced Sea Surface Temperature in the Northwestern Pacific Ocean [PDF]

Sensors
Mesoscale eddies play an important role in upper-ocean heat redistribution, yet the mechanisms controlling eddy-induced sea surface temperature anomaly (SSTA) patterns remain incompletely understood.
Xiangyu Yao, Yunlong Shi
doaj   +2 more sources

Ecomorphological and Age-Related Adaptations in the Tongues of Phocoena dioptrica (Spectacled Porpoise) and Phocoena spinipinnis (Burmeister’s Porpoise) (Phocoenidae: Cetacea) [PDF]

Animals
Vertebrates’ tongues reflect part of their adaptations to diverse feeding strategies, the types of food items they eat, and the environments where they live.
Cleopatra Mara Loza, Carolina Natalia Zanuzzi, Laura Beatriz Andrini, Cecilia Mariana Krmpotic, Alejo Carlos Scarano, Juan Pablo Loureiro, Claudio Gustavo Barbeito, Alfredo Armando Carlini   +7 more
doaj   +2 more sources

Countercurrent distribution of soybean fatty acid methyl esters biosynthetically labeled with H3 and C14

Journal of Lipid Research, 1961
Fatty acids randomly labeled with C14 and with H3 were prepared by culturing soybean plants (a) in the presence of C14O2, and (b) with H3OH in the nutrient medium.
H.J. Dutton, E.P. Jones, C.R. Scholfield, W. Chorney, N.J. Scully   +4 more
doaj   +1 more source

Purification and characterization of phthalanilide-lipid complexes from tissues

Journal of Lipid Research, 1966
What appears to be a new class of phospholipids has been isolated from dog brain in the form of complexes with a substituted phthalanilide. The complexes were extracted by chloroform-methanol and purified by countercurrent distribution in solvent systems
D.W. Yesair, W.I. Rogers, J.T. Funkhouser, C.J. Kensler   +3 more
doaj   +1 more source

Dispersed Mobile-Phase Countercurrent Chromatography

Separations, 2016
Countercurrent distribution based on liquid–liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents.
Timothy Yiu-Cheong Ho, Hong Xue
doaj   +1 more source

Investigation on the Performance of CO₂ Absorption in Ceramic Hollow-Fiber Gas/Liquid Membrane Contactors

Membranes, 2023
The absorption efficiencies of CO2 in ceramic hollow-fiber membrane contactors using monoethanolamine (MEA) absorbent under both cocurrent- and countercurrent-flow operations were investigated theoretically and experimentally; various MEA absorbent flow ...
Chii-Dong Ho, Hsuan Chang, Yu-Han Chen, Thiam Leng Chew, Jui-Wei Ke   +4 more
doaj   +1 more source

Countercurrent distribution of inositol lipids of plant seeds

Journal of Lipid Research, 1969
The inositol lipids of plant seeds consist of phosphatidyl inositol, the phytoglycolipids, and a previously uncharacterized ceramide-phosphate-polysaccharide. These three species have been separated from each other and from the common glycerophosphatides
H.E. Carter, A. Kisic
doaj   +1 more source

Die Trennung von Carotinoidgemischen durch Gegenstromextraktion (II)

CHIMIA, 1973
Countercurrent distribution has been used for the separation of β-carotene and its epoxides without rearrangement reactions. The separation of an extract from Carica papaya shows that this method is suitable for the detection of carotenoids which occur ...
H. Pfander, F. Haller, K. Bernhard, H. Thommen   +3 more
doaj   +1 more source