Countercurrent chromatography of proteins using aqueous two-phase systems [PDF]
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.The biotechnology industry requires high - capacity protein manufacturing processes that retain protein functionality.
Bourton, Emma Catherine
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Advanced modelling and visualisation of liquid-liquid separations of complex sample components, with variable phase distribution and mode of operation [PDF]
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.This research is about liquid-liquid chromatography modelling.
De Folter, Jozefus Johannes Martinus
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Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification. [PDF]
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Wang Z +3 more
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Prediction of Elution Profiles in Countercurrent Chromatography With a Linear Loss of Stationary Phase. [PDF]
A common challenge in reliably predicting elution profiles in countercurrent chromatography using the ProMISE2 software is a persistent loss of stationary phase (i.e., bleeding). This drawback can be overcome by a simple calibration method in the case of linear bleeding.
Rüttler F, Ormos R, Vetter W.
europepmc +2 more sources
The hydrodynamics of countercurrent chromatography in J-type centrifuges [PDF]
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Countercurrent chromatography (CCC) is an advanced liquid-liquid extraction technique that purifies chemical components from complex mixtures.
Wood, Philip Leslie
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Enantioseparation using a counter-current bioreactor [PDF]
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.The potential of countercurrent chromatography (CCC) as a small footprint bioreactor/separator for manufacture of enantiopure chiral molecules was explored,
Grudzien, Lukasz
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Die Fraktionierung von Polymethylmethacrylaten nach dem Molekulargewicht durch Gegenstromverteilung
A polydisperse sample of polymethylmethacrylate with an average molecular weight of 168000 has been fractionated by countercurrent distribution into fractions of different molecular weight using a phase pair prepared from acetone, n-hexane and water.
P. von Tavel, J. Wälchli, H. Rüfenacht
doaj +1 more source
Kinetic modeling of countercurrent saccharification
Background Countercurrent saccharification is a promising way to minimize enzyme loading while obtaining high conversions and product concentrations. However, in countercurrent saccharification experiments, 3–4 months are usually required to acquire a ...
Chao Liang +3 more
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Fatty acids randomly labeled with C14 and with H3 were prepared by culturing soybean plants (a) in the presence of C14O2, and (b) with H3OH in the nutrient medium.
H.J. Dutton +4 more
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Purification and characterization of phthalanilide-lipid complexes from tissues
What appears to be a new class of phospholipids has been isolated from dog brain in the form of complexes with a substituted phthalanilide. The complexes were extracted by chloroform-methanol and purified by countercurrent distribution in solvent systems
D.W. Yesair +3 more
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