Results 81 to 90 of about 30,771 (303)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Research on hydrogen induced cracking behavior and service performance of metal pipeline material
Frontiers in MaterialsThis study systematically investigates the fracture behavior of X80 pipeline steel welded joints under hydrogen-induced cracking (HIC) conditions through combined experimental characterization and numerical simulation.
Yongjun Jia +3 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Prominent Movement Disorders in RNU2‐2‐Related Spliceosomopathy
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Pediatric movement disorders often overlap with neurodevelopmental diseases, suggesting shared molecular mechanisms. Variants in small nuclear RNA (snRNA) genes encoding spliceosome components have recently been associated with neurodevelopmental disorders, termed “RNUopathies.” We analyzed genome sequencing data from 14 patients with ...
Magdalena Krygier +6 more
wiley +1 more source
Crack paths in soft thin sheets
Fracture and Structural Integrity, 2019 Highly deformable materials (elastomers, gels, biological tissues, etc.) are ubiquitous in nature as well as in technology. The understanding of their flaw sensitivity is crucial to ensure a desired safety level.
Roberto Brighenti +2 more
doaj +3 more sources
A Workflow to Accelerate Microstructure‐Sensitive Fatigue Life Predictions
Advanced Engineering Materials, EarlyView.This study introduces a workflow to accelerate predictions of microstructure‐sensitive fatigue life. Results from frameworks with varying levels of simplification are benchmarked against published reference results. The analysis reveals a trade‐off between accuracy and model complexity, offering researchers a practical guide for selecting the optimal ...
Luca Loiodice +2 more
wiley +1 more source
Performance Analysis of Abradable Coating Systems for Aircraft Gas Turbines
Advanced Engineering Materials, EarlyView.Three CoNiCrAlY/YSZ/MgAl2O4 abradable liner configurations on a nickel‐superalloy are evaluated by thermal‐gradient cycling and incursion tests. Laser ablation of the bondcoat and/or Y2O3‐stabilized ZrO2 (YSZ) intermediate layer increases mechanical interlocking and bonding for thick topcoats.
Hanna Heyl +4 more
wiley +1 more source
Advanced Engineering Materials, EarlyView.Mg–Zn composites with a thickness of 0.21 mm were fabricated using roll bonding of a kirigami‐patterned Mg alloy inlay within a Zn matrix. Thermal activation following this process led to the formation of tailored intermetallic structures, which provided the composite with enhanced flexural strength.
Yaroslav Frolov +4 more
wiley +1 more source
Dislocation emission and cleavage process at crack tip [PDF]
, 1998 The competition process between dislocation emission along a pair of symmetric slip planes and quasi-cleavage at Mode I crack tip is investigated in this paper.
Wang, TC (reprint author), Chinese Acad Sci, Inst Mech, LNM, Beijing 100080, Peoples R China. +1 more
core
Study of some optimal XFEM type methods [PDF]
, 2007 The XFEM method in fracture mechanics is revisited. A first improvement is considered using an enlarged fixed enrichment subdomain aroud the crack tip and a bonding condition for the corresponding degree of freedom.
Laborde, Patrick +9 more
core +1 more source