Results 101 to 110 of about 98,107 (317)
Cleavage‐Resistant CYLD Protects Against Autoimmune Hepatitis
Advanced Science, EarlyView.Proteolytic cleavage of the deubiquitinase CYLD emerges as a critical driver of autoimmune hepatitis. TNFα‐induced CYLD loss in macrophages amplifies S100A9‐triggered MAPK activation, leading to excessive chemokine production and hepatic inflammation. Pharmacological inhibition of MEK signaling effectively attenuates experimental disease, highlighting ...
Han Liu +13 more
wiley +1 more source
L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast
Nature Communications, 2018 The International Synthetic Yeast Sc2.0 project has built Cre recombinase sites into synthetic chromosomes, enabling rapid genome evolution. Here the authors demonstrate L-SCRaMbLE, a light-controlled recombinase tool with improved control over ...
Lena Hochrein +4 more
doaj +1 more source
Cell-type specific expression of a dominant negative PKA mutation in mice. [PDF]
PLoS ONE, 2011 We employed the Cre recombinase/loxP system to create a mouse line in which PKA activity can be inhibited in any cell-type that expresses Cre recombinase.
Brandon S Willis +4 more
doaj +1 more source
Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System [PDF]
, 2014 The precise elimination of selectable marker genes is highly desirable, when their function is no longer needed, because their presence raised worldwide public concerns against the release of genetically modified plants.
Bányai, Judit +8 more
core +1 more source
Advanced Science, EarlyView.Elevated exogenous palmitic acid promotes the S‐palmitoylation of SRPK1 in endothelial cells, a dynamic process governed by ZDHHC24 and APT1. This post‐translational modification strengthens the interaction between SRPK1 and the E3 ubiquitin ligase MIB1, thereby facilitating the proteasomal degradation of SRPK1.
Xiao‐Hui Tan +11 more
wiley +1 more source
Unloxing the assembly and activation mechanism of Cre recombinase using cryo-EM [PDF]
, 2021 Kye Stachowski, Mark P. Foster
openalex +1 more source
Simultaneous deletion of floxed genes mediated by CaMKIIa-Cre in the brain and in male germ cells: application to conditional and conventional disruption of Go-alfa [PDF]
, 2014 The Cre/LoxP system is a well-established approach to spatially and temporally control genetic inactivation. The calcium/calmodulin-dependent protein kinase II alpha subunit (CaMKIIα) promoter limits expression to specific regions of the forebrain and ...
Birnbaumer, Lutz +6 more
core +2 more sources
Advanced Science, EarlyView.A muscle‐bone endocrine pathway in aging is revealed in which extracellular vesicles released from atrophic skeletal muscle (Aged‐SKM‐EVs) inhibit bone formation. These EVs deliver miR‐125a‐5p to osteoblasts, thereby suppressing the SIRT7‐Sp7 signaling axis and osteogenic differentiation.
Xiaoyan Shao +22 more
wiley +1 more source
Disease Models & Mechanisms, 2018 Conditional deletion of RBP-J, the major transcriptional effector of Notch signaling, specifically within renin-expressing cells leads to the development of B-cell leukemia.
Brian Chipman Belyea +3 more
doaj +1 more source
Cell type–specific manipulation with GFP-dependent Cre recombinase [PDF]
Nature Neuroscience, 2015 There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split ...
Tang, Jonathan C Y +10 more
openaire +3 more sources