Application of serine integrases for secondary metabolite pathway assembly in Streptomyces [PDF]
Synthetic and Systems Biotechnology, 2020 Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be ...
Hong Gao +2 more
exaly +5 more sources
Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation [PDF]
Nature Communications, 2016 The integron integrases have evolved to perform recombination of single and double stranded DNA. Here the authors show that the ancestral pathway is still functional at double stranded sites, revealing the evolution towards the modern resolution pathway.
JOSÉ Antonio Escudero +2 more
exaly +3 more sources
Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases
Biomolecules, 2021 Integrases of different retroviruses assemble as functional complexes with varying multimers of the protein. Retroviral integrases require a divalent metal cation to perform one-step transesterification catalysis.
Anthony J. Rabe +3 more
doaj +2 more sources
Phage integrases for the construction and manipulation of transgenic mammals
Reproductive Biology and Endocrinology, 2003 Phage integrases catalyze site-specific, unidirectional recombination between two short att recognition sites. Recombination results in integration when the att sites are present on two different DNA molecules and deletion or inversion when the att sites
Sclimenti Christopher R +5 more
doaj +2 more sources
Bracoviruses recruit host integrases for their integration into caterpillar's genome.
PLoS Genetics, 2021 Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown.
Zehua Wang +11 more
doaj +2 more sources
A trade-off between the fitness cost of functional integrases and long-term stability of integrons. [PDF]
PLoS Pathogens, 2012 Horizontal gene transfer (HGT) plays a major role in bacterial microevolution as evident from the rapid emergence and spread of antimicrobial drug resistance.
Irina Starikova +7 more
doaj +2 more sources
Integrase and integration: biochemical activities of HIV-1 integrase [PDF]
Retrovirology, 2008 Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration.
Deprez Eric +4 more
doaj +3 more sources
A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
doaj +2 more sources
Variable orthogonality of serine integrase interactions within the ϕC31 family [PDF]
Scientific ReportsSerine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage ...
Alasdair I. MacDonald +6 more
doaj +2 more sources
Multi‐Targeting Non‐Specific Genome Engineering in Bacteria [PDF]
Advanced ScienceGenome engineering plays a crucial role in the rapidly growing fields of metabolic engineering and synthetic biology. Chromosomal integration and stable expression of functional genes or large metabolic pathways necessitate the development of host ...
Runze Sun +7 more
doaj +2 more sources