Results 21 to 30 of about 10,041 (223)
Metagenomic analysis of lysogeny in Tampa Bay: implications for prophage gene expression. [PDF]
PLoS ONE, 2008 Phage integrase genes often play a role in the establishment of lysogeny in temperate phage by catalyzing the integration of the phage into one of the host's replicons.
Lauren McDaniel +6 more
doaj +1 more source
EMBO reports, 2010 Antiretroviral drugs to prevent integration of the HIV viral genome into chromosomes are undergoing clinical trials, yet they have been developed with an imperfect understanding of their mechanism of action. The recent crystal structure of the major viral protein integrase from a related, little‐known retrovirus, has finally provided insight into how ...
openaire +2 more sources
Resistance to Integrase Inhibitors [PDF]
Viruses, 2010 Integrase (IN) is a clinically validated target for the treatment of human immunodeficiency virus infections and raltegravir exhibits remarkable clinical activity. The next most advanced IN inhibitor is elvitegravir. However, mutant viruses lead to treatment failure and mutations within the IN coding sequence appear to confer cross-resistance.
Mathieu Métifiot +3 more
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Synthesis of programmable integrases [PDF]
Proceedings of the National Academy of Sciences, 2009 Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes ...
Russell M, Gordley +2 more
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Retroviral Integrase: Then and Now [PDF]
Annual Review of Virology, 2015 The retroviral integrases are virally encoded, specialized recombinases that catalyze the insertion of viral DNA into the host cell's DNA, a process that is essential for virus propagation. We have learned a great deal since the existence of an integrated form of retroviral DNA (the provirus) was first proposed by Howard Temin in 1964. Initial studies
Mark D, Andrake, Anna Marie, Skalka
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Retroviral integrases and their cousins [PDF]
Current Opinion in Structural Biology, 1996 The recently determined structures of the catalytic domains of HIV integrase, avian sarcoma virus integrase and the Mu transposase are strikingly similar to each other and also exhibit significant similarity to several nucleases. All these enzymes of cut polynucleotides, leaving 3'OH and 5'PO4 groups. The integrase and transposase also possess a strand-
Rice, Phoebe +2 more
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Frontiers in Microbiology, 2016 In this study, we searched for integrative conjugative elements (ICEs) in chromosomes of 124 strains of Streptococci. These exhaustive analyses revealed 105 putative ICEs and 26 slightly decayed elements that were grouped in 7 distinct families ...
Chloé eAmbroset +13 more
doaj +1 more source
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]
PLoS ONE, 2013 Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi +6 more
doaj +1 more source
Pervasive suicidal integrases in deep-sea archaea
, 2020 Mobile genetic elements often encode integrases which catalyze the site-specific insertion of their genetic information into the host genome and the reverse reaction of excision.
Da Cunha, Violette +3 more
core +1 more source
Positioning Diverse Type IV Structures and Functions Within Class 1 CRISPR-Cas Systems
Frontiers in Microbiology, 2021 Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas nucleases, integrases, and other genetic features commonly observed in most ...
Hannah N. Taylor +6 more
doaj +1 more source