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Mobile DNA, 2011 Background Integrons are genetic elements able to integrate and disseminate genes as cassettes by a site-specific recombination mechanism. These elements contain a gene coding for an integrase that carries out recombination by interacting with two ...
Larouche André, Roy Paul H
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Integrase-On-Demand: Bioprospecting integrases for targeted genomic insertion of genetic cargo [PDF]
Nucleic Acids ResearchABSTRACT Integrases recognize defined sites within DNA and facilitate homologous recombination-independent transfer of genetic fragments. These innate capabilities render integrases as powerful biotechnology tools that mediate efficient site-specific integration of large genetic cargos.
Hannah M McClain +8 more
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Variable orthogonality of serine integrase interactions within the ϕC31 family [PDF]
Scientific ReportsSerine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage ...
Alasdair I. MacDonald +6 more
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Multi‐Targeting Non‐Specific Genome Engineering in Bacteria [PDF]
Advanced ScienceGenome engineering plays a crucial role in the rapidly growing fields of metabolic engineering and synthetic biology. Chromosomal integration and stable expression of functional genes or large metabolic pathways necessitate the development of host ...
Runze Sun +7 more
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Integron and its role in antimicrobial resistance: A literature review on some bacterial pathogens [PDF]
Iranian Journal of Basic Medical Sciences, 2021 In recent years, different acquired resistance mechanisms, including transposons, bacteriophages, plasmids, and integrons have been identified as involved in the spread of resistance genes in bacteria.
Parisa Sabbagh +3 more
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Development of circular AAV cargos for targeted seamless insertion with large serine integrases
Molecular Therapy: Methods & Clinical DevelopmentRecent advancements in gene insertion have shifted from DNA-repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo
Brett J.G. Estes +12 more
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Advances in Biomedical and Health Sciences, 2022 Antibiotic resistance genes (ARGs) are classified as emerging environmental pollutants of global public health concern. These ARGs are disseminated through genetic elements such as integrons.
Anthony Ifeanyin Okoh +1 more
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Use of orthogonal serine integrases to multiplex plasmid conjugation and integration from E. coli into Streptomyces [PDF]
, 2021 Some major producers of useful bioactive natural products belong to the genus Streptomyces or related actinobacteria. Geneticengineering of these bacteria and the pathways that synthesize their valuable products often relies on serine integrases ...
Gao, Hong, Smith, Margaret
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A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. [PDF]
PLoS ONE, 2011 The production of cells capable of expressing gene(s) of interest is important for a variety of applications in biomedicine and biotechnology, including gene therapy and animal transgenesis.
Shigeyuki Yamaguchi +5 more
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Genetic characteristics and integration specificity of Salmonella enterica temperate phages
Frontiers in Microbiology, 2023 IntroductionTemperate phages can engage in the horizontal transfer of functional genes to their bacterial hosts. Thus, their genetic material becomes an intimate part of bacterial genomes and plays essential roles in bacterial mutation and evolution ...
Siqi Sun, Siqi Sun, Xianglilan Zhang
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