There and turn back again: the application of phage serine integrases in eukaryotic systems [PDF]
Frontiers in Bioengineering and BiotechnologySerine integrases (Ints) have gained prominence and have been extensively used in Synthetic Biology due to their ability to modify DNA sequences. Ints are recombinases encoded by the phage genome and have been used to unidirectionally catalyze an ...
Thais Torquato Sales +7 more
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Effect of
Mobile DNA, 2011 Background Integrons are genetic elements able to integrate and disseminate genes as cassettes by a site-specific recombination mechanism. These elements contain a gene coding for an integrase that carries out recombination by interacting with two ...
Larouche André, Roy Paul H
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Development of circular AAV cargos for targeted seamless insertion with large serine integrases
Molecular Therapy: Methods & Clinical DevelopmentRecent advancements in gene insertion have shifted from DNA-repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo
Brett J.G. Estes +12 more
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Integron and its role in antimicrobial resistance: A literature review on some bacterial pathogens [PDF]
Iranian Journal of Basic Medical Sciences, 2021 In recent years, different acquired resistance mechanisms, including transposons, bacteriophages, plasmids, and integrons have been identified as involved in the spread of resistance genes in bacteria.
Parisa Sabbagh +3 more
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Advances in Biomedical and Health Sciences, 2022 Antibiotic resistance genes (ARGs) are classified as emerging environmental pollutants of global public health concern. These ARGs are disseminated through genetic elements such as integrons.
Anthony Ifeanyin Okoh +1 more
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High fidelity one-pot DNA assembly using orthogonal serine integrases [PDF]
, 2023 Background: Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA).
Abioye, J +7 more
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Use of orthogonal serine integrases to multiplex plasmid conjugation and integration from E. coli into Streptomyces [PDF]
, 2021 Some major producers of useful bioactive natural products belong to the genus Streptomyces or related actinobacteria. Geneticengineering of these bacteria and the pathways that synthesize their valuable products often relies on serine integrases ...
Gao, Hong, Smith, Margaret
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Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargo. [PDF]
Nucleic Acids ResABSTRACT Integrases recognize defined sites within DNA and facilitate homologous recombination-independent transfer of genetic fragments. These innate capabilities render integrases as powerful biotechnology tools that mediate efficient site-specific integration of large genetic cargos.
McClain HM +8 more
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Genetic characteristics and integration specificity of Salmonella enterica temperate phages
Frontiers in Microbiology, 2023 IntroductionTemperate phages can engage in the horizontal transfer of functional genes to their bacterial hosts. Thus, their genetic material becomes an intimate part of bacterial genomes and plays essential roles in bacterial mutation and evolution ...
Siqi Sun, Siqi Sun, Xianglilan Zhang
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The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-attB sites in the genus Nocardia [PDF]
PeerJ, 2018 Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited.
Marion Herisse +8 more
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