Application of serine integrases for secondary metabolite pathway assembly in Streptomyces [PDF]
Synthetic and Systems Biotechnology, 2020 Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be ...
Paul Fogg +2 more
exaly +15 more sources
New Applications for Phage Integrases [PDF]
Journal of Molecular Biology, 2014 Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from ϕC31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular ...
Paul Fogg +2 more
exaly +8 more sources
Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation [PDF]
Nature Communications, 2016 The integron integrases have evolved to perform recombination of single and double stranded DNA. Here the authors show that the ancestral pathway is still functional at double stranded sites, revealing the evolution towards the modern resolution pathway.
Didier Mazel +2 more
exaly +3 more sources
Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases
Biomolecules, 2021 Integrases of different retroviruses assemble as functional complexes with varying multimers of the protein. Retroviral integrases require a divalent metal cation to perform one-step transesterification catalysis.
Anthony J. Rabe +3 more
doaj +2 more sources
There and turn back again: the application of phage serine integrases in eukaryotic systems [PDF]
Frontiers in Bioengineering and BiotechnologySerine integrases (Ints) have gained prominence and have been extensively used in Synthetic Biology due to their ability to modify DNA sequences. Ints are recombinases encoded by the phage genome and have been used to unidirectionally catalyze an ...
Elibio Rech, Rayane Nunes Lima
exaly +4 more sources
Phage integrases for the construction and manipulation of transgenic mammals [PDF]
Reproductive Biology and Endocrinology, 2003 Phage integrases catalyze site-specific, unidirectional recombination between two short att recognition sites. Recombination results in integration when the att sites are present on two different DNA molecules and deletion or inversion when the att sites
Sclimenti Christopher R +5 more
doaj +2 more sources
Integrase and integration: biochemical activities of HIV-1 integrase [PDF]
Retrovirology, 2008 Integration of retroviral DNA is an obligatory step of retrovirus replication because proviral DNA is the template for productive infection. Integrase, a retroviral enzyme, catalyses integration.
Deprez Eric +4 more
doaj +3 more sources
Bracoviruses recruit host integrases for their integration into caterpillar's genome.
PLoS Genetics, 2021 Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown.
Zehua Wang +11 more
doaj +2 more sources
Seasonal Occurrence, Population Diversity, and Mobilome Features of Environmental Vibrio parahaemolyticus in Coastal and Estuarine Waters of Haiyan, Zhejiang, China [PDF]
MicroorganismsVibrio parahaemolyticus is a leading cause of seafood-associated gastroenteritis, yet the ecological and genomic significance of environmental populations as potential reservoirs remains incompletely characterized.
Jingyu Xu, Yangang He, Peiyan He
doaj +2 more sources
A trade-off between the fitness cost of functional integrases and long-term stability of integrons. [PDF]
PLoS Pathogens, 2012 Horizontal gene transfer (HGT) plays a major role in bacterial microevolution as evident from the rapid emergence and spread of antimicrobial drug resistance.
Irina Starikova +7 more
doaj +2 more sources