Results 41 to 50 of about 10,041 (223)

Streptavidins Coordinate Biotin Sequestration and Self‐Resistance Within a Biotin‐Pathway Antibiotic Network

Advanced Science, EarlyView.
A conserved genomic region between two streptavidin genes in Streptomyces packages biosynthetic gene clusters for diverse biotin‐pathway antibiotics: acidomycin, stravidin, the new non‐proteinogenic amino acid ANDA, and the new BioA inhibitor α‐methyl‐KAPA.
Sumire Kurosawa, Jingjun Mo, Xiaotong Diao, Feng Xie, Tingting Wang, Haowen Zhao, Dalei Wu, Rolf Müller, Chengzhang Fu   +8 more
wiley   +1 more source

Genomic and Functional Characterization of Vancomycin-Resistant Enterococci-Specific Bacteriophages in the Galleria mellonella Wax Moth Larvae Model

Pharmaceutics, 2022
Phages are naturally occurring viruses that selectively kill bacterial species without disturbing the individual’s normal flora, averting the collateral damage of antimicrobial usage.
Lynn El Haddad, Georgios Angelidakis, Justin R. Clark, Jesus F. Mendoza, Austen L. Terwilliger, Christopher P. Chaftari, Mark Duna, Serena T. Yusuf, Cynthia P. Harb, Mark Stibich, Anthony Maresso, Roy F. Chemaly   +11 more
doaj   +1 more source

Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants

Advanced Science, EarlyView.
A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei, Kai Zhang, Shuke Deng, Jinjie Chen, Xiaomei Huang, Jingfei Guo, Yuqing Wu, Yingjie Guo, Zhen Liang   +8 more
wiley   +1 more source

Multiple choices for HIV therapy with integrase strand transfer inhibitors

Retrovirology, 2012
Two integrases inhibitors, raltegravir and elvitegravir, have now been approved by regulatory agencies for use in the treatment of HIV-infected patients; and the approval of a third such drug, dolutegravir, is expected during 2013 on the basis of several
Raffi Francois, Wainberg Mark A
doaj   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Funcionalidade de serina integrases no controle de interruptores genéticos em plantas [PDF]

, 2020
Tese (doutorado)—Universidade de Brasília, Programa em Rede Multi-Institucional do Pró-Centro-Oeste de Pós-Graduação em Biotecnologia e Biodiversidade, 2020.A biologia sintética tem sido caracterizada por abordagens interdisciplinares voltadas para o ...
Gomide, Mayna da Silveira
core  

Large serine integrases utilise scavenged phage proteins as directionality cofactors. [PDF]

Nucleic Acids Res
Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the directionality of recombination to favour excision over insertion.
Alsaleh A, Holland A, Shin H, Reyes TP, Baksh A, Taiwo-Aiyerin OT, Pigli Y, Rice PA, Olorunniji FJ.   +8 more
europepmc   +3 more sources

RNA‐centric world of retroviruses: unravelling the molecular strategies of genomic RNA packaging

Biological Reviews, EarlyView.
ABSTRACT Retroviruses constitute a unique group of RNA viruses that have profoundly influenced both evolutionary trajectories and biomedical research. Their ability to reverse transcribe and integrate into host genomes has shaped genomic architecture across species and contributed to our understanding of oncogenes, gene regulation, and RNA biology ...
Mohammad Abdullah Jehad, Suresha G. Prabhu, Lizna M. Ali, Vineeta N. Pillai, Farah Mustafa, Tahir A. Rizvi   +5 more
wiley   +1 more source

IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings

Nature Communications, 2023
Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad impacts on gene expression, genome evolution, and the spread of drug-resistance in bacteria.
Mercedes Spínola-Amilibia, Lidia Araújo-Bazán, Álvaro de la Gándara, James M. Berger, Ernesto Arias-Palomo   +4 more
doaj   +1 more source

Biomass‐derived hydrochars as eco‐friendly adsorbents for wastewater treatment applications

Environmental Progress &Sustainable Energy, EarlyView.
Abstract Emerging organic pollutants (EOPs), such as diethyl phthalate (DEP), bisphenol A (BPA), and methylene blue (MB), are only partially removed in conventional wastewater treatment plants. This study assesses hydrochars produced by hydrothermal carbonization (HTC) of spruce bark (SB), vine shoots (VSs), and wheat straw (WSs) for removing three ...
Emanuel Gheorghita Armanu, Sara Ribeirinho‐Soares, Christian Eberlein, Hermann J. Heipieper, Irina Volf, Olga C. Nunes, Olívia Salomé G. P. Soares   +6 more
wiley   +1 more source