Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina
BMC Biotechnology, 2019 Background Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms.
Hong Gao +5 more
doaj +1 more source
RAG-1 Mutations Associated with B-Cell-Negative SCID Dissociate the Nicking and Transesterification Steps of V(D)J Recombination [PDF]
, 2001 Some patients with B-cell-negative severe combined immune deficiency (SCID) carry mutations in RAG-1 or RAG-2 that impair V(D)J recombination. Two recessive RAG-1 mutations responsible for B-cell-negative SCID, R621H and E719K, impair V(D)J recombination
Chang, Fu-Chung +2 more
core +2 more sources
Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer
Nature Communications, 2019 Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ...
Marcus D. Wilson +8 more
doaj +1 more source
Influence of the amino-terminal sequence on the structure and function of HIV integrase
Retrovirology, 2020 Background Antiretroviral therapy (ART) can mitigate the morbidity and mortality caused by the human immunodeficiency virus (HIV). Successful development of ART can be accelerated by accurate structural and biochemical data on targets and their responses
Grant Eilers +7 more
doaj +1 more source
Control of serine integrase recombination directionality by fusion with the directionality factor [PDF]
, 2017 Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL ...
Akopian +43 more
core +3 more sources
Mobile resistome of human gut and pathogen drives anthropogenic bloom of antibiotic resistance [PDF]
, 2020 BACKGROUND:The impact of human activities on the environmental resistome has been documented in many studies, but there remains the controversial question of whether the increased antibiotic resistance observed in anthropogenically impacted environments ...
Bu, Ji-Hye +12 more
core +1 more source
PLoS Pathogens, 2023 Transmissions of simian viruses to humans has originated the different groups of HIV-1. We recently identified a functional motif (CLA), in the C-terminal domain of the integrase, essential for integration in HIV-1 group M.
Elenia Toccafondi +4 more
doaj +2 more sources
Genome integration and excision by a new Streptomyces bacteriophage, ϕJoe [PDF]
, 2017 Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage.
Fogg, Paul C M +3 more
core +2 more sources
Draft Genome Sequence of Mycobacterium heckeshornense Strain RLE. [PDF]
, 2015 We report here the draft genome sequence of Mycobacterium heckeshornense strain RLE isolated from a sputum sample from a patient with shortness of breath.
Chiu, Charles Y +3 more
core +1 more source
Repressing Integrase attachment site operation with CRISPR-Cas9 in E. coli [PDF]
, 2017 Serine integrases are bacteriophage proteins responsible for integrating the phage genome into that of the host. Synthetic biologists have co-opted these proteins into useful tools for permanent DNA logic, utilizing their specific DNA recombination ...
Murray, Richard M., Shur, Andrey
core +1 more source