Results 31 to 40 of about 10,473 (233)
High fidelity one-pot DNA assembly using orthogonal serine integrases [PDF]
, 2023 Background: Large serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA).
Abioye, J +7 more
core +3 more sources
The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-attB sites in the genus Nocardia [PDF]
PeerJ, 2018 Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited.
Marion Herisse +8 more
doaj +2 more sources
Metagenomic analysis of lysogeny in Tampa Bay: implications for prophage gene expression. [PDF]
PLoS ONE, 2008 Phage integrase genes often play a role in the establishment of lysogeny in temperate phage by catalyzing the integration of the phage into one of the host's replicons.
Lauren McDaniel +6 more
doaj +1 more source
Applications of Serine Integrases in Synthetic Biology over the Past Decade
, 2023 Serine integrases are emerging as one of the most powerful biological tools for biotechnology. Over the past decade, many research papers have been published on the use of serine integrases in synthetic biology.
Luyao Wang +4 more
core +1 more source
Frontiers in Microbiology, 2016 In this study, we searched for integrative conjugative elements (ICEs) in chromosomes of 124 strains of Streptococci. These exhaustive analyses revealed 105 putative ICEs and 26 slightly decayed elements that were grouped in 7 distinct families ...
Chloé eAmbroset +13 more
doaj +1 more source
Resistance to Integrase Inhibitors [PDF]
Viruses, 2010 Integrase (IN) is a clinically validated target for the treatment of human immunodeficiency virus infections and raltegravir exhibits remarkable clinical activity. The next most advanced IN inhibitor is elvitegravir. However, mutant viruses lead to treatment failure and mutations within the IN coding sequence appear to confer cross-resistance.
Métifiot, Marchand, Maddali, Pommier
openaire +3 more sources
Synthesis of programmable integrases [PDF]
Proceedings of the National Academy of Sciences, 2009 Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes ...
Russell M, Gordley +2 more
openaire +2 more sources
Retroviral Integrase: Then and Now [PDF]
Annual Review of Virology, 2015 The retroviral integrases are virally encoded, specialized recombinases that catalyze the insertion of viral DNA into the host cell's DNA, a process that is essential for virus propagation. We have learned a great deal since the existence of an integrated form of retroviral DNA (the provirus) was first proposed by Howard Temin in 1964. Initial studies
Mark D, Andrake, Anna Marie, Skalka
openaire +2 more sources
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]
PLoS ONE, 2013 Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi +6 more
doaj +1 more source
Positioning Diverse Type IV Structures and Functions Within Class 1 CRISPR-Cas Systems
Frontiers in Microbiology, 2021 Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas nucleases, integrases, and other genetic features commonly observed in most ...
Hannah N. Taylor +6 more
doaj +1 more source