Results 31 to 40 of about 13,851 (226)
Frontiers in Microbiology, 2016 In this study, we searched for integrative conjugative elements (ICEs) in chromosomes of 124 strains of Streptococci. These exhaustive analyses revealed 105 putative ICEs and 26 slightly decayed elements that were grouped in 7 distinct families ...
Chloé eAmbroset +13 more
doaj +1 more source
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]
PLoS ONE, 2013 Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi +6 more
doaj +1 more source
Transcriptome analysis of bacteriophage communities in periodontal health and disease. [PDF]
, 2015 BackgroundThe role of viruses as members of the human microbiome has gained broader attention with the discovery that human body surfaces are inhabited by sizeable viral communities.
Abeles, Shira R +5 more
core +2 more sources
Positioning Diverse Type IV Structures and Functions Within Class 1 CRISPR-Cas Systems
Frontiers in Microbiology, 2021 Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas nucleases, integrases, and other genetic features commonly observed in most ...
Hannah N. Taylor +6 more
doaj +1 more source
A trade-off between the fitness cost of functional integrases and long-term stability of integrons.
PLoS Pathogens, 2012 Horizontal gene transfer (HGT) plays a major role in bacterial microevolution as evident from the rapid emergence and spread of antimicrobial drug resistance.
Irina Starikova +7 more
doaj +1 more source
Cluster J Mycobacteriophages: Intron Splicing in Capsid and Tail Genes [PDF]
, 2013 Bacteriophages isolated on Mycobacterium smegmatis mc2155 represent many distinct genomes sharing little or no DNA sequence similarity. The genomes are architecturally mosaic and are replete with genes of unknown function.
Best, AA +17 more
core +4 more sources
Mechanistic modelling of a recombinase-based two-input temporal logic gate [PDF]
, 2017 Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Bates, Declan +3 more
core +1 more source
Serine integrase chimeras with activity in E. coli and HeLa cells
Biology Open, 2014 In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj +1 more source
Synthesis of programmable integrases [PDF]
Proceedings of the National Academy of Sciences, 2009 Accurate modification of the 3 billion-base-pair human genome requires tools with exceptional sequence specificity. Here, we describe a general strategy for the design of enzymes that target a single site within the genome. We generated chimeric zinc finger recombinases with cooperative DNA-binding and catalytic specificities that integrate transgenes ...
Russell M, Gordley +2 more
openaire +2 more sources
Resistance to Integrase Inhibitors [PDF]
Viruses, 2010 Integrase (IN) is a clinically validated target for the treatment of human immunodeficiency virus infections and raltegravir exhibits remarkable clinical activity. The next most advanced IN inhibitor is elvitegravir. However, mutant viruses lead to treatment failure and mutations within the IN coding sequence appear to confer cross-resistance.
Mathieu Métifiot +3 more
openaire +3 more sources