Control of serine integrase recombination directionality by fusion with the directionality factor. [PDF]
Nucleic Acids Res, 2017 Bacteriophage serine integrases are extensively used in biotechnology and synthetic biology for assembly and rearrangement of DNA sequences. Serine integrases promote recombination between two different DNA sites, attP and attB, to form recombinant attL ...
Olorunniji FJ +5 more
europepmc +13 more sources
SYMBIOSIS: synthetic manipulable biobricks via orthogonal serine integrase systems. [PDF]
Nucleic Acids Res, 2022 Serine integrases are emerging as one of the most powerful biological tools for synthetic biology. They have been widely used across genome engineering and genetic circuit design.
Ba F, Liu Y, Liu WQ, Tian X, Li J.
europepmc +7 more sources
Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination. [PDF]
PLoS Genetics, 2013 Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Shweta Singh +2 more
doaj +11 more sources
Variable orthogonality of serine integrase interactions within the ϕC31 family [PDF]
Scientific ReportsSerine integrases are phage- (or mobile element-) encoded enzymes that catalyse site-specific recombination reactions between a short DNA sequence on the phage genome (attP) and a corresponding host genome sequence (attB), thereby integrating the phage ...
Alasdair I. MacDonald +6 more
doaj +6 more sources
Serine integrase chimeras with activity in E. coli and HeLa cells [PDF]
Biology Open, 2014 In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj +6 more sources
Mathematical model of a serine integrase-controlled toggle switch with a single input. [PDF]
J R Soc Interface, 2018 Dual-state genetic switches that can change their state in response to input signals can be used in synthetic biology to encode memory and control gene expression.
Pokhilko A +3 more
europepmc +8 more sources
Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination. [PDF]
Nucleic Acids Res, 2014 Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase.
Colloms SD +7 more
europepmc +12 more sources
Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase [PDF]
BMC BiotechnologyBackground Mammalian display is an appealing technology for therapeutic antibody development. Despite the advantages of mammalian display, such as full-length IgG display with mammalian glycosylation and its inherent ability to select antibodies with ...
Olli Huhtinen +4 more
doaj +3 more sources
Protocol for the establishment of a serine integrase-based platform for functional validation of genetic switch controllers in eukaryotic cells. [PDF]
PLoS ONESerine integrases (Ints) are a family of site-specific recombinases (SSRs) encoded by some bacteriophages to integrate their genetic material into the genome of a host.
Marco A de Oliveira +13 more
doaj +5 more sources
Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination. [PDF]
J Bacteriol, 2021 The robust and exquisitely regulated site-specific recombination reactions promoted by serine integrases are integral to the life cycle of temperate bacteriophage and, in the case of the A118 prophage, are an important virulence factor of Listeria ...
Mandali S, Johnson RC.
europepmc +2 more sources