Application of serine integrases for secondary metabolite pathway assembly in Streptomyces [PDF]
Synthetic and Systems Biotechnology, 2020 Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be ...
Hong Gao +4 more
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Making serine integrases work for us [PDF]
Current Opinion in Microbiology, 2017 DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements. Serine integrases are a group of recombinases derived from bacteriophages.
Stark, W. Marshall, W Marshall Stark
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Serine Integrase-Based Event Recording in E. coli [PDF]
, 2022 DNA is a unique molecule that has evolved to serve as the genetic material for life. It seems straightforward to consider this molecule not only as a wonder of the natural world but as a tool for information storage and retrieval. Bacteria have evolved to conserve DNA, but bacteriophages have evolved to specifically integrate their genomes using ...
Shur, Andrey Sergeyvich
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Structural basis of unidirectional DNA recombination by the ϕC31 serine integrase [PDF]
Abstract Large serine integrases catalyse the integration and excision of bacteriophage (phage) DNA genomes into and from the genomes of their bacterial hosts by site-specific recombination 1 .
Sun YE +5 more
europepmc +3 more sources
Tight regulation of the intS gene of the KplE1 prophage: a new paradigm for integrase gene regulation. [PDF]
PLoS Genetics, 2010 Temperate phages have the ability to maintain their genome in their host, a process called lysogeny. For most, passive replication of the phage genome relies on integration into the host's chromosome and becoming a prophage.
Gaël Panis +5 more
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Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS ONE, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Lin Zhang +4 more
doaj +2 more sources
The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-attB sites in the genus Nocardia [PDF]
PeerJ, 2018 Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited.
Marion Herisse +8 more
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Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination. [PDF]
PLoS ONE, 2010 BACKGROUND: Amino acid sequence alignment of phage phiC31 integrase with the serine recombinases family revealed highly conserved regions outside the catalytic domain. Until now, no system mutational or biochemical studies have been carried out to assess
Shaohui Liu +7 more
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GCN2 phosphorylates HIV-1 integrase and decreases HIV-1 replication by limiting viral integration [PDF]
Scientific Reports, 2017 GCN2 is a serine/threonine kinase involved in cellular stress response related to amino acid starvation. Previously, we showed that GCN2 interacts with HIV-1 integrase and is activated during HIV-1 infection.
A. Jaspart +13 more
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Serine integrase recombinational engineering (SIRE): A versatile toolbox for genome editing
Biotechnology and Bioengineering, 2018 AbstractChromosomal integration of biosynthetic pathways for the biotechnological production of high‐value chemicals is a necessity to develop industrial strains with a high long‐term stability and a low production variability. However, the introduction of multiple transcription units into the microbial genome remains a difficult task.
Nico Snoeck +9 more
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