Results 21 to 30 of about 217,395 (184)

Development of circular AAV cargos for targeted seamless insertion with large serine integrases

Molecular Therapy: Methods & Clinical Development
Recent advancements in gene insertion have shifted from DNA-repair-dependent mechanisms to more precise approaches, enhancing safety and predictability for editing outcomes. Integrase-mediated programmable genomic integration (I-PGI) utilizes a DNA cargo
Brett J.G. Estes, Nisha Gandhi, Jessica R. Von Stetina, Dev Paudel, Angela X. Nan, Parth Amin, Joshua Rose, Shuai Wu, Kangni Zheng, Yijun Zhang, Jesse C. Cochrane, Jonathan D. Finn, Jenny Xie   +12 more
doaj   +3 more sources

Making serine integrases work for us [PDF]

Current Opinion in Microbiology, 2017
DNA site-specific recombinases are enzymes (often associated with mobile DNA elements) that catalyse breaking and rejoining of DNA strands at specific points, thereby bringing about precise genetic rearrangements.
Stark, W. Marshall
core   +4 more sources

Mechanistic modelling of a recombinase-based two-input temporal logic gate [PDF]

Engineering Biology, 2017
Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Jack E. Bowyer, Victoria Hsiao, Wilson W. Wong, Declan G. Bates   +3 more
doaj   +3 more sources

Tetrameric structure of a serine integrase catalytic domain. [PDF]

Structure, 2008
The serine integrases have recently emerged as powerful new chromosome engineering tools in various organisms and show promise for therapeutic use in human cells. The serine integrases are structurally and mechanistically unrelated to the bacteriophage lambda integrase but share a similar catalytic domain with the resolvase/invertase enzymes typified ...
P. Yuan, Kushol Gupta, G. V. Van Duyne
semanticscholar   +5 more sources

S-SELeCT: a human-evolved serine integrase system for efficient large-cargo genome integration. [PDF]

Nucleic Acids Res
As a consequence of their sizes, many loss-of-function genetic mutations fall within large genes. A major gene-therapy tool that could be used to solve large swaths of the genetic diseases that result from these inherited mutations is large-fragment ...
Farruggio AP, Jiang L, Duong K, Nguyen C, Kaddoura R, Tsai R.   +5 more
europepmc   +2 more sources

Application of serine integrases for secondary metabolite pathway assembly in Streptomyces [PDF]

Synthetic and Systems Biotechnology, 2020
Serine integrases have been shown to be efficient tools for metabolic pathway assembly. To further improve the flexibility and efficiency of pathway engineering via serine integrases, we explored how multiple orthogonally active serine integrases can be ...
Hong Gao, Gabrielle Taylor, Stephanie K. Evans, Paul C.M. Fogg, Margaret C.M. Smith   +4 more
doaj   +4 more sources

Serine Integrases: Advancing Synthetic Biology [PDF]

ACS Synthetic Biology, 2018
Serine integrases catalyze precise rearrangement of DNA through site-specific recombination of small sequences of DNA called attachment (att) sites. Unlike other site-specific recombinases, the recombination reaction driven by serine integrases is highly directional and can only be reversed in the presence of an accessory protein called a recombination
Christine A. Merrick, Jia Zhao, Susan J. Rosser   +2 more
openaire   +5 more sources

Structural basis of DNA recombination catalysis and regulation by ϕC31 integrase

bioRxiv
Large serine integrases catalyse the integration and excision of bacteriophage (phage) DNA genomes into and from the genomes of their bacterial hosts by site-specific recombination1.
Sun YE, Aspinall L, Joseph AP, Colloms SD, Stark WM, Spagnolo L.   +5 more
europepmc   +2 more sources

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology [PDF]

Microorganisms, 2019
Recent advances in synthetic biology and an emerging algal biotechnology market have spurred a prolific increase in the availability of molecular tools for cyanobacterial research.
Grant A. R. Gale, Alejandra A. Schiavon Osorio, Lauren A. Mills, Baojun Wang, David J. Lea-Smith, Alistair J. McCormick   +5 more
doaj   +4 more sources

Integration and excision by the large serine recombinase φRv1 integrase [PDF]

Molecular Microbiology, 2005
SummaryThe Mycobacterium tuberculosis prophage‐like element φRv1 encodes a site‐specific recombination system utilizing an integrase of the serine recombinase family. Recombination occurs between a putative attP site and the host chromosome, but is unusual in that the attB site lies within a redundant repetitive element (REP13E12)  of  which  there ...
Lori A, Bibb, Maria I, Hancox, Graham F, Hatfull   +2 more
openaire   +4 more sources