Results 1 to 10 of about 279,397 (214)

Genome‐scale engineering for systems and synthetic biology

Molecular Systems Biology, 2013
Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome.
Kevin M Esvelt, Harris H Wang
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Systematic optimization of prime editing for enhanced efficiency and versatility in genome engineering across diverse cell types [PDF]

Frontiers in Cell and Developmental Biology
Prime editing offers remarkable versatility in genome editing, but its efficiency remains a major bottleneck. While continuous optimization of the prime editing enzymes and guide RNAs (pegRNAs) has improved editing outcomes, the method of delivery also ...
Huiling Mu, Huiling Mu, Yeyi Liu, Yeyi Liu, Yijia Chi, Yijia Chi, Fei Wang, Shuting Meng, Shuting Meng, Yi Zhang, Yi Zhang, Xunting Wang, Xunting Wang, Dongxin Zhao, Dongxin Zhao, Dongxin Zhao   +15 more
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CRISPR/Cas-Mediated Genome Engineering in Plants: Application and Prospectives [PDF]

Plants
Genetic engineering has become an essential element in developing climate-resilient crops and environmentally sustainable solutions to respond to the increasing need for global food security.
Swetaleena Mishra, Subhendu Nayak, Narendra Tuteja, Sowmya Poosapati, Durga Madhab Swain, Ranjan Kumar Sahoo   +5 more
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Isolation, Characterization, and Genome Engineering of a Lytic Pseudomonas aeruginosa Phage [PDF]

Microorganisms
Antibiotic-resistant bacterial infections have become one of the leading causes of human mortality. Bacteriophages presented great potential for combating antibiotic-resistant infections in the post-antibiotic era due to their high host specificity and ...
Xiaomei Cong, Shuang Zhao, Qing Zhang, Shuo Liu, Youming Zhang, Fu Yan   +5 more
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Recent advances in genome-scale engineering in Escherichia coli and their applications

Engineering Microbiology
Owing to the rapid advancement of genome engineering technologies, the scale of genome engineering has expanded dramatically. Genome editing has progressed from one genomic alteration at a time that could only be employed in few species, to the ...
Hui Gao, Zhichao Qiu, Xuan Wang, Xiyuan Zhang, Yujia Zhang, Junbiao Dai, Zhuobin Liang   +6 more
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The Rice Serine/Arginine Splicing Factor RS33 Regulates Pre-mRNA Splicing during Abiotic Stress Responses

Cells, 2022
Abiotic stresses profoundly affect plant growth and development and limit crop productivity. Pre-mRNA splicing is a major form of gene regulation that helps plants cope with various stresses.
Haroon Butt, Jeremie Bazin, Kasavajhala V. S. K. Prasad, Nourelislam Awad, Martin Crespi, Anireddy S. N. Reddy, Magdy M. Mahfouz   +6 more
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Gene editing tools for mycoplasmas: references and future directions for efficient genome manipulation

Frontiers in Microbiology, 2023
Mycoplasmas are successful pathogens that cause debilitating diseases in humans and various animal hosts. Despite the exceptionally streamlined genomes, mycoplasmas have evolved specific mechanisms to access essential nutrients from host cells.
Gang Zhao, Gang Zhao, Gang Zhao, Doukun Lu, Min Li, Min Li, Yujiong Wang, Yujiong Wang   +7 more
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A Method for Electroporation of Cre Recombinase Protein into Intact Nicotiana tabacum Cells

Plants, 2023
The Cre/lox recombination system has become a powerful technology for gene function analysis in a broad spectrum of cell types and organisms. In our previous report, Cre protein had been successfully delivered into intact Arabidopsis thaliana cells using
Yuichi Furuhata, Emiko Egi, Tomi Murakami, Yoshio Kato   +3 more
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A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling

eLife, 2020
Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms.
Deepti Trivedi, Vinitha CM, Karishma Bisht, Vishnu Janardan, Awadhesh Pandit, Bishal Basak, Shwetha H, Navyashree Ramesh, Padinjat Raghu   +8 more
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Investigation of Genome Biology by Synthetic Genome Engineering

Bioengineering, 2023
Synthetic genomes were designed based on an understanding of natural genomic information, offering an opportunity to engineer and investigate biological systems on a genome-wide scale. Currently, the designer version of the M.
Hui Zhang, Yao Xiong, Wenhai Xiao, Yi Wu
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