Results 11 to 20 of about 279,397 (214)
Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule
Frontiers in Plant Science, 2017 The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR).
Haroon Butt +8 more
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MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells
Viruses, 2019 The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics.
Kapil Gupta +5 more
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Multiplexed CRISPR-Cas9-Based Genome Editing of
mSphere, 2019 Microbial production of biofuels and bioproducts offers a sustainable and economic alternative to petroleum-based fuels and chemicals. The basidiomycete yeast Rhodosporidium toruloides is a promising platform organism for generating bioproducts due to ...
Peter B. Otoupal +5 more
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CRISPR‐TAPE: protein‐centric CRISPR guide design for targeted proteome engineering
Molecular Systems Biology, 2020 Rational molecular engineering of proteins with CRISPR‐based approaches is challenged by the gene‐centric nature of gRNA design tools. To address this, we have developed CRISPR‐TAPE, a protein‐centric gRNA design algorithm that allows users to target ...
Daniel Paolo Anderson +3 more
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Biological Parts for Kluyveromyces marxianus Synthetic Biology
Frontiers in Bioengineering and Biotechnology, 2019 Kluyveromyces marxianus is a non-conventional yeast whose physiology and metabolism lends itself to diverse biotechnological applications. While the wild-type yeast is already in use for producing fragrances and fermented products, the lack of ...
Arun S. Rajkumar +4 more
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Genome Editing in Trees: From Multiple Repair Pathways to Long-Term Stability
Frontiers in Plant Science, 2018 The CRISPR technology continues to diversify with a broadening array of applications that touch all kingdoms of life. The simplicity, versatility and species-independent nature of the CRISPR system offers researchers a previously unattainable level of ...
William Patrick Bewg +3 more
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iPSCs in NK Cell Manufacturing and NKEV Development
Frontiers in Immunology, 2022 Natural killer (NK) cell immunotherapies for cancer can complement existing T cell therapies while benefiting from advancements already made in the immunotherapy field.
Nicholas Boyd-Gibbins +4 more
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Frontiers in Chemistry, 2014 Incorporating non-standard amino acids (NSAAs) into proteins enables new chemical properties, new structures, and new functions. In recent years, improvements in cell-free protein synthesis (CFPS) systems have opened the way to accurate and efficient ...
Seok Hoon eHong +2 more
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Application of Gene Editing Technology in Resistance Breeding of Livestock
Life, 2022 As a new genetic engineering technology, gene editing can precisely modify the specific gene sequence of the organism’s genome. In the last 10 years, with the rapid development of gene editing technology, zinc-finger nucleases (ZFNs), transcription ...
Sutian Wang +8 more
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Induction of gametocytogenesis in human malaria parasites: from stress to genome editing
Frontiers in MicrobiologyGametocytogenesis is a crucial process in which malaria parasites transition from their asexual stage to the sexual-stage gametocytes. This transformation enables the parasite to infect and multiply within the Anopheles mosquito, the vector responsible ...
Rishitharan Subramaniam +4 more
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