Results 1 to 10 of about 427,199 (265)
Microbial Cell Factories, 2022 Background Membrane proteins (MPs) are an important class of molecules with a wide array of cellular functions and are part of many metabolic pathways.
Chiara Guidi +6 more
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Microbial Cell Factories, 2022 Background The rapidly expanding synthetic biology toolbox allows engineers to develop smarter strategies to tackle the optimization of complex biosynthetic pathways.
Maarten Van Brempt +6 more
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A synthetic transcription platform for programmable gene expression in mammalian cells
Nature Communications, 2022 Precise and scalable regulation of gene expression in mammalian cells is challenging. Here, the authors created a highly tunable CRISPR-based synthetic transcription system for programmable control of mammalian gene expression and cellular activity.
William C. W. Chen +13 more
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“Cell-Free Synthetic Biology”: Synthetic Biology Meets Cell-Free Protein Synthesis
Methods and Protocols, 2019 Since Nirenberg and Matthaei used cell-free protein synthesis (CFPS) to elucidate the genetic code in the early 1960s [...]
Seok Hoon Hong
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A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
Nature Communications, 2018 Efficient biosynthesis of lactams is still undesirable due to lacking of suitable enzyme. Here, the authors develop a sensitive transcription factor-based biosensor for high-throughput screening of marine metagenome and find a cyclase that can cyclize ω ...
Soo-Jin Yeom +10 more
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Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
BMC Plant Biology, 2018 Background Isoprene is a five-carbon chemical that is an important starting material for the synthesis of rubber, elastomers, and medicines. Although many plants produce huge amounts of isoprene, it is very difficult to obtain isoprene directly from ...
Soo-Jin Yeom +8 more
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Inducible expression of large gRNA arrays for multiplexed CRISPRai applications
Nature Communications, 2022 CRISPR gene activation and inhibition has become a powerful synthetic tool for influencing the expression of native genes for foundational studies, cellular reprograming, and metabolic engineering.
William M. Shaw +7 more
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Synthetic biology, engineering biology, market expectation
Engineering Biology, 2020 ‘Engineering biology’ is being increasingly adopted as a term by organisations that seek to deliver benefits from ‘synthetic biology’. However, are ‘engineering biology’ and ‘synthetic biology’ different words with the same meaning or do they signal ...
Lionel Clarke
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Nature CommunicationsSynthetic biology allows us to reuse, repurpose, and reconfigure biological systems to address society’s most pressing challenges. Developing biotechnologies in this way requires integrating concepts across disciplines, posing challenges to educating ...
Ashty S. Karim +16 more
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Progression in the In Vitro Macrophage Expansion
Journal of Immunology ResearchMacrophages play essential roles in homeostasis and disease, and they were considered terminally differentiated cells that cannot proliferate. However, growing evidence shows that macrophages can self-renew in homeostasis and multiple pathological states
Yunpeng Wei +4 more
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